Nature 351, 122 - 127 (09 May 1991); doi:10.1038/351122a0

Ha-Ras augments c-Jun activity and stimulates phosphorylation of its activation domain

Bernard Binétruy^*†, Tod Smeal^*‡ & Michael Karin^*§

^*Departments of Pharmacology and
^‡Biology, Center for Molecular Genetics, School of Medicine, 0636, University of California San Diego, La Jolla, California 92093, USA
^†Present address: Institut Curie-Biologie, Centre Universitaire, 91405 Orsay, France
^§To whom correspondence should be addressed

Ha-Ras augments c-Jun-mediated transactivation by potentiating the activity of the c-Jun activation domain. Ha-Ras also causes a corresponding increase in phosphorylation of specific sites in that part of the c-Jun protein. A Ha-Ras-induced protein kinase cascade resulting in hyperphosphorylation of the c-Jun activation domain could explain how these oncoproteins cooperate to transform rat embryo fibroblasts.

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