Nature 351, 117 - 121 (09 May 1991); doi:10.1038/351117a0

Male development of chromosomally female mice transgenic for Sry

Peter Koopman, John Gubbay, Nigel Vivian, Peter Goodfellow^* & Robin Lovell-Badge^†

Laboratory of Eukaryotic Molecular Genetics, MRC National Institute for Medical Research, The Ridgeway, Mill Hill, London NW7 1AA, UK
^*Human Molecular Genetics Laboratory, Imperial Cancer Research Fund, PO Box 123, Lincoln's Inn Fields, London WC2A 3PX, UK
^†To whom correspondence should be addressed

The initiation of male development in mammals requires one or more genes on the Y chromosome. A recently isolated gene, termed SRY in humans and Sry in mouse, has many of the genetic and biological properties expected of a Y-located testis-determining gene. It is now shown that Sry on a 14-kilobase genomic DNA fragment is sufficient to induce testis differentiation and subsequent male development when introduced into chromosomally female mouse embryos.

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