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Article
Nature 350, 398 - 402 (04 April 1991); doi:10.1038/350398a0

Primary structure and functional expression from complementary DNA of a brain calcium channel

Yasuo Mori*, Thomas Friedrich*, Man-Suk Kim*, Atsushi Mikami*, Junichi Nakai*, Peter Ruth, Eva Bosse, Franz Hofmann, Veit Flockerzi, Teiichi Furuichi§, Katsuhiko Mikoshiba§, Keiji Imoto*, Tsutomu Tanabe* & Shosaku Numa*

* Departments of Medical Chemistry and Molecular Genetics, Kyoto University Faculty of Medicine, Kyoto 606, Japan Institut für Medizinische Biochemie, Medizinische Fakultät, Universität des Saarlandes, D-6650 Homburg/Saar, Germany § Division of Behavior and Neurobiology, National Institute for Basic Biology, Okazaki 444, Japan Division of Regulation of Macromolecular Function, Institute for Protein Research, Osaka University, Suita 565, Japan Present address: BASF AG, ZHB/P A30, D-6700 Ludwigshafen, Germany.
To whom correspondence should be addressed.

The primary structure of a voltage-dependent cal-cium channel from rabbit brain has been deduced by cloning and sequencing the complementary DNA. Calcium channel activity expressed from the cDNA is dramatically increased by coexpression of the α2 and β subunits, known to be associated with the dihydropyridine receptor. This channel is a high voltage-activated calcium channel that is insensitive both to nifedipine and to ω-conotoxin. We suggest that it is expressed predominantly in cerebellar Purkinje cells and granule cells.

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