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Letters to Nature
Nature 345, 60 - 63 (03 May 1990); doi:10.1038/345060a0

Genetic diversity in Sargasso Sea bacterioplankton

Stephen J. Giovannoni, Theresa B. Britschgi, Craig L. Moyer & Katharine G. Field

Department of Microbiology, Oregon State University, Corvallis, Oregon 97331, USA

BACTERIOPLANKTON are recognized as important agents of biogeochemical change in marine ecosystems, yet relatively little is known about the species that make up these communities. Uncertainties about the genetic structure and diversity of natural bacterioplankton populations stem from the traditional difficulties associated with microbial cultivation techniques. Discrepancies between direct counts and plate counts are typically several orders of magnitude, raising doubts as to whether cultivated marine bacteria are actually representative of dominant planktonic species1–3. We have phylogenetically analysed clone libraries of eubacterial 16S ribosomal RNA genes amplified from natural populations of Sargasso Sea picoplankton by the polymerase chain reaction4. The analysis indicates the presence of a novel microbial group, the SAR 11 cluster, which appears to be a significant component of this oligotrophic bacterioplankton community. A second cluster of lineages related to the oxygenic phototrophs—cyanobacteria, prochlorophytes and chloroplasts—was also observed. However, none of the genes matched the small subunit rRNA sequences of cultivated marine cyanobacteria from similar habitats. The diversity of 16S rRNA genes observed within the clusters suggests that these bacterioplankton may be consortia of independent lineages sharing surprisingly distant common ancestors.

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References

1. Jannasch, H. W. & Jones, G. E. Limnol. Oceanogr. 4, 128−139 (1959).
2. Ferguson, R. L. & Rublee, P. Limnol. Oceanogr. 21, 141−145 (1976).
3. Kogure, K., Simidu, U. & Taga, N. Can. J. Microbiol. 25, 415−420 (1979). | PubMed | ISI | ChemPort |
4. Saiki, R. K. et al. Science 239, 487−491 (1988). | PubMed | ISI | ChemPort |
5. Menzel, D. W. & Ryther, J. H. Deep-Sea Res. 6, 351−367 (1959).
6. Gutell, R. R. et al. Prog. Nucleic Acid Res. molec. Biol. 32, 155−216 (1985). | ChemPort |
7. Shuldiner, A., Nirula, A. & Roth, J. Nucleic Acids Res. 17, 4409 (1989). | PubMed | ChemPort |
8. Giovannoni, S. J. et al. J. Bact. 170, 3584−3592 (1988). | PubMed | ChemPort |
9. Waterbury, J. B., Watson, S. W., Valois, F. W. & Franks, D. G. Can. Bull. Fish. Aquat. Sci. 214, 71−120 (1987).
10. Waterbury, J. B., Willey, J. M., Franks, D. G., Valois, F. W. & Watson, S. W. Science 230, 74−76 (1985).
11. Woese, C. R., Stackebrandt, E., Macke, T. J. & Fox, G. E. System. appl. Microbiol. 6, 143−152 (1985). | ChemPort |
12. Woese, C. Microbiol. Rev. 51, 221−271 (1987). | PubMed | ISI | ChemPort |
13. Giovannoni, S. J., DeLong, E., Olsen, G. J. & Pace, N. R. J. Bact. 170, 720−726 (1988). | PubMed | ChemPort |
14. Sogin, M. L., Pechman, K. J., Zablen, L., Lewis, B. J. & Woese, C. R. J. Bact. 112, 13−22 (1972). | PubMed | ChemPort |
15. Dunning, A. M., Talmud, P. & Humphries, S. E. Nucleic Acids Res. 16, 10393 (1988). | PubMed | ChemPort |
16. Ho, S. et al. Gene 77, 51−59 (1989). | Article | PubMed | ISI | ChemPort |
17. Selander, R. K., Caugant, D. A. & Whittam, T. S. in Escherichia coli and Salmonella typhimurium, Cellular, Molecular Biology (eds Neidhardt, F. C. et al.) 1625−1648 (American Society for Microbiology, Washington, 1987).
18. McArthur, J. V., Kovacic, D. A. & Smith, M. H. Proc. natn. Acad. Sci. U.S.A. 85, 9621−9624 (1988). | ChemPort |
19. Proctor, L. M. & Fuhrman, J. A. Nature 343, 60−61 (1990). | Article | ISI |
20. Wayne, L. G. et al. Int. J. system. Bact. 37, 463−464 (1987).
21. Olsen, G. J., Lane, D. J., Giovannoni, S. J., Pace, N. R. & Stahl, D. A. A. Rev. Microbiol. 40, 337−365 (1985).
22. Sanger, F., Nicklen, S. & Coulsen, A. R. Proc. natn. Acad. Sci. U.S.A. 74, 5463−5467 (1977). | ChemPort |
23. Jukes, T. H. & Cantor, C. R. in Mammalian Protein Metabolism (ed. Munro, H. N.) 21 (Academic, New York, 1969). | ChemPort |
24. Kimura, M. & Ohta, T. J. molec. Evol. 2, 87−90 (1972). | PubMed | ISI | ChemPort |
25. Olsen, G. J. Meth. Enzym. 164, 793−812 (1988). | PubMed | ChemPort |
26. Scharf, S. J., Horn, G. T. & Ehrlich, H. A. Science 233, 1076−1078 (1986). | PubMed | ISI | ChemPort |
27. Brosius, J., Dull, T. J., Sleeter, D. D. & Noller, H. F. J. molec. Biol. 148, 107−127 (1981). | Article | PubMed | ISI | ChemPort |
28. Turner, S., Burger-Wiersma, T., Giovannoni, S. J., Mar, L. R. & Pace, N. R. Nature 337, 380−382 (1989). | Article | PubMed | ChemPort |
29. Stackebrandt, E. et al. Arch. Microbiol. 149, 547−556 (1988). | Article | PubMed | ChemPort |
30. Woese, C. R., Debrunner-Vossbrinck, B., Oyaizu, H., Stackebrandt, E. & Ludwig, W. Science 229, 762−765 (1985). | PubMed | ChemPort |



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