Abstract
The eukaryotic upstream binding factor (DBF), recognizes the ribosomal RNA gene promoter and activates transcription mediated by RNA poly-merase I through cooperative interactions with the species-specific factor, SL1. Isolation of complementary DNA clones and sequence analysis reveals similarities between DNA binding domains of human UBF (hUBF) and high mobility group (HMG) protein 1. Expression, cellular localization and in vitro transcription studies establish that cloned hUBF encodes a nucleolar factor that binds specifically to the upstream control element and core of the rRNA gene promoter to activate transcription in a binding site-dependent manner.
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References
Mitchell, P. J. & Tjian, R. Science 245, 371–378 (1989).
Johnson, P. F. & McKnight, S. L. A. Rev. Biochem. 58, 799–839 (1989).
Saltzman, A. G. & Weinman, R. FASEB J. 3, 1723–1733 (1989).
Sollner-Webb, B. & Tower, J. A. Rev. Biochem. 55, 801–830 (1986).
Smale, S. T. & Tjian, R. Molec. cell. Biol. 5, 352–362 (1985).
Haltiner, M. M., Smale, S. T. & Tjian, R. Molec. cell. Biol. 6, 227–235 (1986).
Jones, M. H., Learned, R. M. & Tjian, R. Proc. natn. Acad. Sci. U.S.A. 85, 669–673 (1988).
Learned, R. M., Cordes, S. & Tjian, R. Molec. cell. Biol. 5, 1358–1369 (1985).
Learned, R. M., Learned, T. K., Haltiner, M. M. & Tjian, R. T. Cell 45, 847–857 (1986).
Bell, S. P., Learned, R. M., Jantzen, H.-M. & Tjian, R. Science 241, 1192–1197 (1988).
Pikaard, C. S., McStay, B., Schultz, M. C., Bell, S. P. & Reeder, R. H. Genes Dev. 3, 1779–1788 (1989).
Bell, S. P., Pikaard, C. S., Reeder, R. H. & Tjian, R. Cell 59, 489–497 (1989).
Dunaway, M. Genes Dev. 3, 1768–1778 (1989).
Courey, A. J., Holtzman, D. A., Jackson, S. P. & Tjian, R. Cell 59, 827–836 (1989).
Ptashne, M. Nature 335, 683–689 (1988).
Courey, A. J. & Tjian, R. Cell 55, 887–898 (1988).
Mermod, N., O'Neill, E. A., Kelly, T. J. & Tjian, R. Cell 58, 741–753 (1989).
Einck, L. & Bustin, M. Expl cell Res. 156, 295–310 (1985).
Wright, J. M. & Dixon, G. H. Biochemistry 27, 576–581 (1988).
Herr, W. et al. Genes Dev. 2, 1513–1516 (1988).
He, K. et al. Nature 340, 35–42 (1989).
Sturm, R. A. & Herr, W. Nature 336, 601–604 (1988).
Roeder, G. S., Beard, C., Smith, M. & Keranen, S. Molec. cell. Biol. 5, 1543–1553 (1985).
Turner, R. & Tjian, R. Science 243, 1689–1694 (1989).
Kadonaga, J. T., Jones, K. A. & Tjian, R. Trends biochem. Sci. 11, 20–23 (1986).
Carballo, M., Puigdomenech, P. & Palau, J. EMBO J. 2, 1759–1764 (1983).
Earnshaw, W. C. J. Cell. Biol. 105, 1479–1482 (1987).
Tsuda, K., Kikuchi, M., Mori, K., Waga, S. & Yoshida, M. Biochemistry 27, 6159–6163 (1988).
Bernues, J., Espel, E. & Querol, E. Biochim. biophys. Acta 866, 242–251 (1986).
Tremethick, D. J. & Molloy, P. L. J. biol. Chem. 261, 6986–6992 (1986).
Watt, F. & Molloy, P. L. Nucleic. Acids Res. 16, 1471–1486 (1988).
Yang-Yen, H.-F. & Rothblum, L. I. Molec. cell. Biol. 8, 3406–3414 (1988).
Lapeyre, B., Bourbon, H. & Amalric, F. Proc. natn. Acad. Sci. U.S.A. 84, 1472–1476 (1987).
Schmidt-Zachmann, M. S., Hugle-Dorr, B. & Franke, W. W. EMBO J. 6, 1881–1890 (1987).
Kuenzel, E. A., Mulligan, J. A., Sommercorn, J. & Krebs, E. G. J. biol. Chem. 262, 9136–9140 (1987).
Siomi, H. et al. Cell 55, 197–209 (1988).
Bohmann, D. et al. Science 283, 1386–1392 (1987).
Lathe, R. J. molec. Biol. 183, 1–12 (1985).
Kadonaga, J. T., Carner, K. R., Masiarz, F. R. & Tjian, R. Cell 51, 1079–1090 (1987).
Chakrabarti, S., Brechling, K. & Moss, B. Molec. cell. Biol. 5, 3403–3409 (1985).
Dignam, J. D., Lebovitz, R. M. & Roeder, R. G. Nucleic Acids Res. 11, 1475–1489 (1983).
Norman, C., Runswick, M., Pollock, R. & Treisman, R. Cell 55, 989–1003 (1988).
Harlow, E. & Lane, D. Antibodies: A Laboratory Manual (Cold Spring Harbor Laboratory, New York, 1988).
Jackson, S. P. & Tjian, R. Cell 55, 125–133 (1988).
Bell, S. P., Jantzen, H.-M. & Tjian, R. Genes Dev. (in the press).
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Jantzen, HM., Admon, A., Bell, S. et al. Nucleolar transcription factor hUBF contains a DNA-binding motif with homology to HMG proteins. Nature 344, 830–836 (1990). https://doi.org/10.1038/344830a0
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DOI: https://doi.org/10.1038/344830a0
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