Nature 342, 396 - 401 (23 November 1989); doi:10.1038/342396a0

The barrier to recombination between Escherichia coli and Salmonella typhimurium is disrupted in mismatch-repair mutants

Christiane Rayssiguier^*, David S. Thaler^† & Miroslav Radman^*‡

^* Institut Jacques Monod, 2 Place Jussieu, Tour 43, 75251 Paris-Cedex 05, France
^† Biology Department, University of Utah, Salt Lake City, Utah 84112, USA
^‡ Laboratory of Molecular Genetics, National Institute of Environmental Health Sciences, Research Triangle Park, North Carolina 27709, USA

The requirement for DNA sequence homology in generalized genetic recombination is greatly relaxed in bacterial mutL,mutS and mutH mutants deficient in mismatch repair. In such mutants, inter-generic recombination occurs efficiently between Escherichia coli and Salmonella typhimurium, which are 20% divergent in DNA sequence. This finding has implications for speciation, for regulating recombination between diverged repeated sequences, and for hitherto difficult interspecies hybridizations.

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