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Letters to Nature

Nature 335, 721-726 (20 October 1988) | doi:10.1038/335721a0; Accepted 9 September 1988

Resolution of quantitative traits into Mendelian factors by using a complete linkage map of restriction fragment length polymorphisms

Andrew H. Paterson*, Eric S. Lander†‡, John D. Hewitt§, Susan Peterson*, Stephen E. Lincoln† & Steven D. Tanksley*

  1. *Department of Plant Breeding and Biometry, Cornell University, Ithaca, New York 14853, USA
  2. †Whitehead Institute for Biomedical Research, 9 Cambridge Center, Cambridge, Massachusetts 02142, USA
  3. ‡Harvard University, Cambridge, Massachusetts 02138, USA
  4. §Department of Vegetable Crops, University of California, Davis, California 95616, USA
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The conflict between the Mendelian theory of participate inheritance1 and the observation of continuous variation for most traits in nature was resolved in the early 1900s by the concept that quantitative traits can result from segregation of multiple genes, modified by environmental effects2–5. Although pioneering experiments6–9 showed that linkage could occasionally be detected to such quantitative trait loci (QTLs), accurate and systematic mapping of QTLs has not been possible because the inheritance of an entire genome could not be studied with genetic markers7. The use of restriction fragment length polymorphisms10 (RFLPs) has made such investigations possible, at least in principle. Here, we report the first use of a complete RFLP linkage map to resolve quantitative traits into discrete Mendelian factors, in an interspecific back-cross of tomato. Applying new analytical methods, we mapped at least six QTLs controlling fruit mass, four QTLs for the concentration of soluble solids and five QTLs for fruit pH. This approach is broadly applicable to the genetic dissection of quantitative inheritance of physiological, morphological and behavioural traits in any higher plant or animal.