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Telomeric repeat from T. thermophila cross hybridizes with human telomeres

Nature volume 332, pages 656–659 (1988)Cite this article

Abstract

The ends (telomeres) of eukaryotic chromosomes must have special features to ensure their stability and complete replication. Studies in yeast1–3, protozoa4–6, slime moulds7,8 and flagellates8,10 show that telomeres are tandem repeats of simple sequences that have a G-rich and a C-rich strand. Mammalian telomeres have yet to be isolated and characterized, although a DNA fragment within 20 kilobases of the telomeres of the short arms of the human sex chromosomes has been isolated11. Recently we showed that a chromosome from the fission yeast Schizosaccharomyces pombe could, in some cases, replicate as an autonomous mini-chromosome in mouse cells12. By extrapolation from other systems1,3,13,14, we reasoned that mouse telomeres could be added to the S. pombe chromosome ends in the mouse cells. On setting out to test this hypothesis we found to our surprise that the telomeric probe used (containing both the S. pombe and Tetrahymena thermophila repeats) hybridized to a series of discrete fragments in normal mouse DNA and DNA from a wide range of eukaryotes. We show here that the sequences hybridizing to this probe are located at the telomeres of most, if not all, human chromosomes and are similar to the Tetrahymena telomeric-repeat component of the probe.

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References

  1. Shampay, J., Szostak, J. W. & Blackburn, E. H. Nature 310, 154–157

  2. Walmsley, R. W., Chan, C. S. M., Tye, B. K. & Petes, T. D. Nature 310, 157–160 (1984).

    Article  ADS  CAS  Google Scholar 

  3. Sugawara, N. & Szostak, J. W. Yeast 2 (supp.) 373 (1986).

    Google Scholar 

  4. Blackburn, E. H. & Gall, J. G. J. molec. Biol. 120, 33–53 (1978).

    Article  CAS  Google Scholar 

  5. Klobutcher, L. A., Swanton, M. A., Donini, P. & Prestcott, D. M. Proc. natn. Acad. Sci. U.S.A. 78, 3015–3019 (1981).

    Article  ADS  CAS  Google Scholar 

  6. Ponzi, M., Pace, T., Dore, E. & Frontali, C. EMBO. J. 4, 2991–2995 (1985).

    Article  CAS  Google Scholar 

  7. Johnson, E. M. Cell 22, 875–886 (1980).

    Article  CAS  Google Scholar 

  8. Emery, H. S. & Weiner, A. M. Cell 26, 411–419 (1981).

    Article  CAS  Google Scholar 

  9. Blackburn, E. H. & Challoner, P. B. Cell 36, 447–457 (1984).

    Article  CAS  Google Scholar 

  10. Van der Ploeg, L. H. T., Lin, A. Y. C. & Borst, P. Cell 36, 459–468 (1984).

    Article  CAS  Google Scholar 

  11. Cooke, H. J., Brown, W. R. A. & Rappold, G. A. Nature 317, 687–692 (1985).

    Article  ADS  CAS  Google Scholar 

  12. Allshire, R. C. et al. Cell 50, 391–403 (1987).

    Article  CAS  Google Scholar 

  13. Pluta, A. F., Dani, G. M., Spear, B. B. & Zakian, V. A. Proc. Acad. Sci. U.S. A. 78, 1475–1479 (1984).

    Article  ADS  Google Scholar 

  14. Perrot, M., Barreau, C. & Bégueret, J. Molec. cell. Biol. 7, 1725–1730 (1987).

    Article  CAS  Google Scholar 

  15. Cooke, H. J. & Smith, B. A. Cold Spring Harbor Symp. Quant. Biol. 51, 213–219 (1986).

    Article  CAS  Google Scholar 

  16. Meehan, R. R. et al. Am. J. hum. Genet. 42, 26–37 (1988).

    CAS  PubMed  PubMed Central  Google Scholar 

  17. Gosden, J. R., Middleton, P. G., Rout, D. & De Angelis, C. Cytogenet. Cell Genet. 43, 150–153 (1986).

    Article  CAS  Google Scholar 

  18. Liou, G. I. et al. Somat. Cell. molec. Genet. 13, 315–323 (1987).

    Article  CAS  Google Scholar 

  19. Larson, D. D., Spangler, E. A. & Blackburn, E. H. Cell 50, 477–483 (1987).

    Article  CAS  Google Scholar 

  20. Bernards, A., Michels, P. A. M., Lincke, C. R. & Borst, P. Nature 303, 592–547 (1983).

    Article  ADS  CAS  Google Scholar 

  21. Pays, E., Laurent, M., De Linte, K., Van Meirvenne, N. & Steinert, M. Nucleic Acids Res. 11, 8137–8147 (1983).

    Article  CAS  Google Scholar 

  22. De Grouchy, J., Turleau, C., Finaz, C. & Roubin, M. Chromosome Variation in Human Evolution (ed. Boyce, A. J.) 17–38 (Taylor & Francis, London, 1975).

    Google Scholar 

  23. Lejeune, J., Dutrillaux, B., Rethoré, M. O. & Prieur, M. Chromosoma 43, 423–444 (1973).

    Article  CAS  Google Scholar 

  24. Yunis, J. J. & Prakash, O. Science 215, 1525–1530 (1982).

    Article  ADS  CAS  Google Scholar 

  25. Sutherland, G. R. & Hecht, F. Oxford Monographs on Medical genetics 13, (Oxford University Press, 1985).

    Google Scholar 

  26. Henderson, E. et al. Cell 51, 899–908 (1987).

    Article  CAS  Google Scholar 

  27. Greider, C. W. & Blackburn, E. H. Cell 51, 887–898 (1987).

    Article  CAS  Google Scholar 

  28. Gilliam, T. C. et al. Cell 50, 565–571 (1987).

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. MRC Clinical and Population Cytogenetics Unit, Western General Hospital, Edinburgh, EH4 2XU, UK

    Robin C. Allshire, John R. Gosden, Sally H. Cross, Gwen Cranston, Derek Rout & Nicholas D. Hastie

  2. Massachusetts General Hospital, Boston, Massachusetts, 02114, USA

    Neal Sugawara & Jack W. Szostak

  3. Department of Zoology, University of Edinburgh, Edinburgh, EH9 3JT, UK

    Peter A. Fantes

Authors
  1. Robin C. Allshire
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  2. John R. Gosden
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  3. Sally H. Cross
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  4. Gwen Cranston
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  5. Derek Rout
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  6. Neal Sugawara
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  7. Jack W. Szostak
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  8. Peter A. Fantes
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  9. Nicholas D. Hastie
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Allshire, R., Gosden, J., Cross, S. et al. Telomeric repeat from T. thermophila cross hybridizes with human telomeres. Nature 332, 656–659 (1988). https://doi.org/10.1038/332656a0

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