Abstract
Changing the surface charge of subtilisin by site-directed mutagenesisproduces enzymes with significantly shifted pH-activity profiles, higher catalytic activities and altered specificities. Insight into water as a dielectric, the role of ions in electrostatic shielding and the field effects on catalysis is obtained and suggests rules for tailoring pH-activity profiles.
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Russell, A., Fersht, A. Rational modification of enzyme catalysis by engineering surface charge. Nature 328, 496–500 (1987). https://doi.org/10.1038/328496a0
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DOI: https://doi.org/10.1038/328496a0
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