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Agrobacterium-mediated delivery of infectious maize streak virus into maize plants

Abstract

Cells of certain strains of Agrobacterium colonize plants by transferring a portion of their DNA (the T-DNA) into a host plant cell, so causing it to proliferate and produce substances (opines) which the bacteria can use as food1. Most dicotyledonous plants can act as hosts, but most monocotyledonous species (including the economically important gramineae) are thought not to be susceptible2. We have used agroinfection (Agrobacterium-mediated virus infection3) as a very sensitive assay to test whether DNA transfer at least occurs during Agrobacterium inoculations of maize, a graminaceous plant. Naked DNA of the geminivirus maize streak virus (MSV) is not infectious to plants by itself and the intact virus can only infect if transmitted by an insect vector4. We report here that whole maize plants develop symptoms of viral infection if inoculated with strains of Agrobacterium carrying tandemly repeated copies of MSV genomes in their T-DNA. Mutant Agrobacterium strains defective in transfer of T-DNA cannot transmit MSV DNA in this test. We conclude that cloned MSV DNA is biologically active and that Agrobacterium can transfer DNA to maize.

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References

  1. Gheysen, G., Dhaese, P., Van Montagu, M. & Schelt, J. in Genetic Flux in Plants (eds Hohn B. & Dennis, E. S.) 12–27 (Springer, New York, 1985).

    Google Scholar 

  2. DeCleene, M. Phytopath, Z. 113, 81–89 (1985).

    Article  Google Scholar 

  3. Grimsley, N. & Bisaro, D. in Plant DNA Infectious Agents (eds Hohn, T. & Schell, J.) (Springer, New York, in the press).

  4. Davies, J. W., Townsend, R. & Stanley, J. in Plant DNA Infectious Agents (eds Hohn, T. & Schell, J.) (Springer, New York, in the press).

  5. Stachel, S. E., Messens, E., Van Montagu, M. & Schell, J. Nature 318, 624–629 (1985).

    Article  ADS  Google Scholar 

  6. Bolton, G. W., Nester, E. W. & Gordon, M. P. Science 232, 983–985 (1986).

    Article  ADS  CAS  PubMed  Google Scholar 

  7. Chilton, M-D., Saiki, R. K., Yadav, N., Gordon, M. P. & Quetier, F. Proc. natn. Acad. Sci. U.S.A. 77, 4060–4064 (1980).

    Article  ADS  CAS  Google Scholar 

  8. Gardner, R. & Knauf, V., Science 231, 725–727 (1985).

    Article  ADS  Google Scholar 

  9. Grimsley, N., Hohn, B., Hohn, T. & Walden, R. Proc. natn. Acad. Sci. U.S.A. 83, 3282–3286 (1986).

    Article  ADS  CAS  Google Scholar 

  10. Rogers, S. G. et al. Cell 45, 593–600 (1986).

    Article  CAS  PubMed  Google Scholar 

  11. Mullineaux, P. M., Donson, J., Morris-Krsinich, B. A. M., Boulton, M. I. & Davies, J. W. EMBO J. 3, 3063–3068 (1984).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  12. Howell, S. Nucleic Acids Res. 12, 7359–7375 (1978).

    Article  Google Scholar 

  13. Klee, H. J., White, F. F., Iyer, V. N., Gordon, M. P. & Nester, E. W. J. Bact. 153, 878–883 (1983).

    CAS  PubMed  PubMed Central  Google Scholar 

  14. Bock, K. R., Guthrie, E. J. & Woods, R. D. Ann. appl. Biol. 77, 289–296 (1974).

    Article  CAS  Google Scholar 

  15. Hernalsteens, J. P., Thia-Toong, L., Schell, J. & Van Montagu, M. EMBO J. 3, 3039–3042 (1984).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  16. Hooykaas-Van Slogteren, G. M. S., Hooykaas, P. J. J. & Schilperoort, R. A. Nature 311, 763–764 (1984).

    Article  ADS  CAS  Google Scholar 

  17. Graves, A. C. F. & Goldman, S. L. Plant molec. Biol. 7, 43–50 (1986).

    Article  CAS  Google Scholar 

  18. Wang, K., Herrera-Estrella, L., Van Montagu, M. & Zambryski, P. Cell 38, 455–462 (1984).

    Article  CAS  PubMed  Google Scholar 

  19. Grimsley, N., Hohn, T. & Hohn, B. EMBO J. 5, 641–646 (1986).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  20. Koukolíková-Nicola, Z. et al. Nature 313, 191–196 (1985).

    Article  ADS  Google Scholar 

  21. Stachel, S. E., Timmerman, B. & Zambryski, P. Nature 322, 706 (1986).

    Article  ADS  CAS  Google Scholar 

  22. Potrykus, I., Saul, M. W., Petruska, J., Paszkowski, J. & Shillito, R. D. Molec. gen. Genet. 199, 183–188 (1985).

    Article  CAS  Google Scholar 

  23. Lörz, H., Baker, B. & Shell, J. Molec. gen. Genet. 199, 178–182 (1985).

    Article  Google Scholar 

  24. Fromm, M. E., Taylor, L. P. & Walbot, V. Nature 319, 791–793 (1986).

    Article  ADS  CAS  PubMed  Google Scholar 

  25. Uchimiya, H. et al. Molec. gen. Genet. 204, 204–207 (1986).

    Article  CAS  Google Scholar 

  26. Chang, A. C. Y. & Cohen, S. N. J. Bact. 134, 1141–1146 (1978).

    CAS  PubMed  PubMed Central  Google Scholar 

  27. Birnboim, H. C. & Doly, J. Nucleic Acids Res. 7, 1513–1523 (1979).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  28. Rothstein, S. J. et al. Gene (in the press).

  29. Bevan, M. Nucleic Acids Res. 12, 8711–8721 (1984).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  30. Holsters, M. et al. Plasmid 3, 212–230.

  31. Rogers, S. G., Horsch, R. B. & Fraley, R. T. Meth. Enzym. 118, 627–640 (1986).

    Article  CAS  Google Scholar 

  32. Dhaese, P. et al. Nucleic Acids Res. 7, 1837–1849 (1979).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

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Grimsley, N., Hohn, T., Davies, J. et al. Agrobacterium-mediated delivery of infectious maize streak virus into maize plants. Nature 325, 177–179 (1987). https://doi.org/10.1038/325177a0

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