Nature 320, 695 - 699 (24 April 1986); doi:10.1038/320695a0

cDNA sequence and chromosomal localization of human platelet-derived growth factor A-chain and its expression in tumour cell lines

Christer Betsholtz^*, Ann Johnsson^†, Carl-Henrik Heldin^†**, Bengt Westermark^*, Peter Lind^‡**, Mickey S. Urdea^§, Roger Eddy, Thomas B. Shows, Karen Philpott^¶, Andrew L. Mellor^¶, Timothy J. Knott^£ & James Scott^£

^*Department of Pathology, University Hospital, S-751 85 Uppsala, Sweden
^†Department of Medical and Physiological Chemistry, Box 575, Biomedicum, S-751 23 Uppsala, Sweden
^‡Wallenberg Laboratory, University of Uppsala, Box 562, S-751 22 Uppsala, Sweden
^§Chiron Corporation, 4560 Horton Street, Emeryville, California 94608, USA
Roswell Park Memorial Institute, Department of Human Genetics, Buffalo, New York 14263, USA
^¶Transplantation Biology Division and ^£Molecular Medicine Research Group, MRC Clinical Research Centre, Watford Road, Harrow, Middlesex HA1 3UJ, UK
^**Present addresses: Ludwig Institute for Cancer Research, Biomedicum, 5-751 23 Uppsala, Sweden (C.-H.H.); KabiGen AB, Strandbergsgatan 49, S-112 87 Stockholm, Sweden (P.L.).

The amino-acid sequence of the precursor of the human tumour cell line-derived platelet-derived growth factor (PDGF) A-chain has been deduced from complementary DNA clones and the gene localized to chromosome 7. The protein shows extensive homology to the PDGF B-chain precursor. Expression of the PDGF A-chain gene is independent of that of the PDGF B-chain in a number of human tumour cell lines, and secretion of a PDGF-like growth factor of relative molecular mass 31,000 correlates with expression of A- but not B-chain messenger RNA.

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