Nature 319, 153 - 154 (09 January 1986); doi:10.1038/319153a0

Murine cells expressing an HLA molecule are specifically lysed by HLA-restricted antiviral human T cells

E. Gomard^*, B. Begue^*, S. Sodoyer^†, J. L. Maryanski^‡, B. R. Jordan^† & J. P. Levy

^*INSERM, U 152, Bâtiment Gustave Roussy, Hôpital Cochin, 27 rue du Faubourg Saint-Jacques, 75014 Paris, France
^†Centre d'Immunologie, INSERM-CNRS de Marseille-Luminy, Case 906, Marseille Cedex 9, France
^‡Ludwig Institute for Cancer Research, Brussels Branch, 74 Avenue Hippocrate, UCL 7459, B-1200, Brussels, Belgium

Class I HLA (histocompatibility locus antigen) molecules are the targets of allospecific cytolytic T lymphocytes (CTL) in graft rejection, and constitute the restricting elements necessary for the interaction between antiviral CTL and virus-infected cells. Cells expressing only one HLA in the absence of other human molecules would provide a remarkable model for studying the function of these molecules. However, HLA⁺ murine cells transfected with human genes¹⁻⁵ are generally not lysed by allospecific human CTL⁵⁻⁸, and this is ascribed to insufficient HLA expression, lack of human ₂-microglobulin, alteration of HLA molecules or absence of receptors for human T8 or LFA1 molecules in murine cells⁶⁻⁸. Here we report, for the first time, the specific lysis of virus-infected HLA⁺ murine cells by HLA-restricted antiviral human CTL. Therefore, these murine cells constitute an excellent model for studying the role of HLA molecules.

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