Nature 319, 150 - 153 (09 January 1986); doi:10.1038/319150a0

IgE-mediated degranulation of mast cells does not require opening of ion channels

M. Lindau^* & J. M. Fernandez

Department of Membrane Biophysics, Max-Planck-Institut für biophysikalische Chemie, D-3400 Göttingen, FRG
^*Present address: Biophysics Group, Department of Physics, Freie Universität Berlin, D-1000 Berlin 33, FRG.

Rat peritoneal mast cells respond to antigenic stimulation by releasing histamine through exocytosis. The dynamics of exocytosis can be investigated by dialysing single cells with patch pipettes using the whole-cell recording configuration of the patch-clamp technique^1,2. However, dialysed cells fail to respond to external stimuli such as compound 48/80 or antigens, suggesting that essential cytoplasmic components have been washed out. We have developed a new patch-clamp configuration in which the patch under the pipette tip is not disrupted but instead permeabilized, preventing the diffusion of large molecules out of the cell. In this configuration the cell responds to external stimulation, and the capacitance as well as the conductance of the cell membrane can be recorded during degranulation. On antigenic stimulation, the cell capacitance (proportional to plasma membrane area), after an initial delay, increases by a factor of about 3. This increase in capacitance is often preceded by a transient increase in conductance. Agents that block Ca-activated channels inhibit this conductance change without affecting the amplitude and time course of degranulation. We therefore conclude that, in contrast to excitable secretory cells such as chromaffin cells², mast cells do not use ion channels in stimulus-secretion coupling.

References

1.	Fernandez, J. M., Neher, E. & Gomperts, B. D. Nature 312, 453−455 (1984). | PubMed | ISI | ChemPort |
2.	Neher, E. & Marty, A. Proc. natn. Acad. Sci. U.S.A. 79, 6712−6716 (1982). | ChemPort |
3.	Bennet, J. P., Cockcroft, S. & Gomperts, B. D. J. Physiol., Land. 317, 335−345 (1981).
4.	Lindau, M. & Fernandez, J. M. J. gen. Physiol. (submitted).
5.	Hamill, O. P., Marty, A., Neher, E., Sakmann, B. & Sigworth, F. J. Pflügers Arch. ges. Physiol. 391, 85−100 (1981). | ChemPort |
6.	Fernandez, J. M., Bezanilla, F. & Taylor, R. E. J. gen. Physiol. 79, 41−67 (1982). | Article | PubMed | ISI | ChemPort |
7.	Fishman, M. C. & Spector, I. Proc. natn. Acad. Sci. U.S.A. 78, 5245−5249 (1981). | ChemPort |
8.	Hoffman, E. K., Simonsen, L. O. & Lambert, I. H. J. Membrane Biol. 78, 211−222 (1984).
9.	Gomperts, B. D. Nature 306, 64−66 (1983). | PubMed | ISI | ChemPort |
10.	Mazurek, N., Schindler, H., Schuerholz, T.H. & Pecht, I. Proc. natn. Acad. Sci. U.S.A. 81, 6841−6845 (1984). | ChemPort |
11.	Foreman, J. C., Hallet, M. B. & Mongar, J. I. J. Physiol., Lond. 271, 193−214 (1977). | PubMed | ISI | ChemPort |
12.	Foreman, J. C. & Mongar, J. L. J. Physiol., Lond. 224, 753−769 (1972). | PubMed | ISI | ChemPort |
13.	Grosman, N. & Diamant, B. Trends Pharmac. Sci. 5, 183−184 (1984). | Article | ISI | ChemPort |
14.	White, J. R., Ishizaka, T., Ishizaka, K. & Sha'afi, R. Proc. natn. Acad. Sci. U.S.A. 81, 3978−3982 (1984). | ChemPort |
15.	Ennis, M., Ind, P. W., Pearce, F. L. & Dollery, C. T. Ag. Actions 13, 144−148 (1983). | ISI | ChemPort |