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Article
Nature 318, 538 - 543 (12 December 1985); doi:10.1038/318538a0

Role of acetylcholine receptor subunits in gating of the channel

Bert Sakmann, Christoph Methfessel, Masayoshi Mishina, Tomoyuki Takahashi*, Toshiyuki Takai, Masaaki Kurasaki, Kazuhiko Fukuda & Shosaku Numa

Max-Planck-Institut für biophysikalische Chemie, D-3400 Göttingen, FRG
Departments of Medical Chemistry and Molecular Genetics and *Physiology, Kyoto University Faculty of Medicine, Kyoto 606, Japan

The Torpedo and calf acetylcholine receptors and hybrids composed of subunits from the two species have been produced in Xenopus oocytes by the use of the cloned complementary DNAs. Single-channel current measurements indicate that these receptors form channels of similar conductance but with different gating behaviour.

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