Nature 318, 334 - 338 (28 November 1985); doi:10.1038/318334a0

Topology of signal recognition particle receptor in endoplasmic reticulum membrane

Leander Lauffer^*, Pablo D. Garcia^*, Richard N. Harkins^†, Lisa Coussens^†, Axel Ullrich^† & Peter Walter^*

^*Department of Biochemistry and Biophysics, University of California, San Francisco, California 94143, USA
^†Departments of Molecular Biology and Protein Biochemistry, Genentech, Inc., 460 Point San Bruno Boulevard, South San Francisco, California 94080, USA

The signal recognition particle (SRP) receptor is an integral membrane protein of the endoplasmic reticulum which, in conjunction with SRP, ensures the correct targeting of nascent secretory proteins to this membrane system. From the complementary DNA sequence we have deduced the complete primary structure of the SRP receptor and established that its amino-terminal region is anchored in the membrane. The anchor fragment and the cytoplasmic fragment contribute jointly to a functionally important region which is highly charged and may function in nucleic acid binding.

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