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Nature 317, 782 - 786 (31 October 1985); doi:10.1038/317782a0

The three-dimensional structure of trp repressor

Richard W. Schevitz, Zdzislaw Otwinowski, Andrzej Joachimiak, Catherine L. Lawson & Paul B. Sigler

Department of Biochemistry and Molecular Biology, The University of Chicago, 920 East 58th Street, Chicago, Illinois 60637, USA

The crystal structure of the Escherichia coli trp repressor has been solved to atomic resolution. The dimeric protein has a remarkable subunit interface in which five of each subunit's six helices are interlinked. The binding of L-tryptophan activates the aporepressor indirectly by fixing the orientation of the second helix of the helix–turn–helix motif and by moulding the details of the repressor's structure near the DNA binding surface.

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References

1. Zurawski, G., Gunsalus, R. P., Brown, K. D. & Yanofsky, C. J. molec. Biol. 145, 47−73 (1981). | PubMed | ISI | ChemPort |
2. Kelley, R. L. & Yankofsky, C. Proc. natn. Acad. Sci. U.S.A. 79, 3120−3124 (1982). | ChemPort |
3. Joachimiak, A. J., Kelley, R. L., Gunsalus, R. P., Yanofsky, C. & Sigler, P. B. Proc. natn. Acad. Sci. U.S.A. 80, 668−672 (1983). | ChemPort |
4. Haydock, P. V. & Somerville, R. L. Biochem. biophys. Res. Commun. 119, 926−932 (1984). | PubMed | ISI | ChemPort |
5. Joachimiak, A., Schevitz, R. W., Kelley, R. L., Yanofsky, C. & Sigler, P. B. J. biol. Chem. 258, 12641−12643 (1983). | PubMed | ChemPort |
6. Anderson, W. F., Ohlendorf, D. H., Takeda, Y. & Matthews, B. W. Nature 290, 754−758 (1981). | PubMed | ISI | ChemPort |
7. Pabo, C. O. & Lewis, M. Nature 298, 443−497 (1982). | PubMed | ISI | ChemPort |
8. McKay, D. B. & Steitz, T. A. Nature 290, 744 (1981). | PubMed | ISI | ChemPort |
9. Tsapakos, M. J., Haydock, P. V., Hermodson, M. & Somerville, R. L. J. biol. Chem. (in the press).
10. Kelley, R. L. & Yanofsky, C. Proc. natn. Acad. Sci. U.S.A. 82, 483−487 (1985). | ChemPort |
11. Hendrickson, W. A. & Konnert, J. H. in Biomolecular Structure, Conformation and Evolution Vol. 1 (ed. Srinivassan, R.) 43−57 (Pergamon, Oxford, 1980).
12. Jones, T. A. in Computational Crystallography (ed. Sayre, D.) 303−317 (Clarendon, Oxford, 1982).
13. Gunsalus, R. P. & Yanofsky, C. Proc. natn. Acad. Sci. U.S.A. 77, 7117−7121 (1980). | ChemPort |
14. Herzberg, O. & James, M. N. G. Nature 313, 653−659 (1985). | PubMed | ISI | ChemPort |
15. Sundaralingam, M. et al. Science 227, 945−948 (1985). | PubMed | ISI | ChemPort |
16. Babu, Y. S. et al. Nature 315, 37−40 (1985). | PubMed | ISI | ChemPort |
17. Ohlendorf, D. H., Anderson, W. F., Lewis, M., Pabo, C. O. & Matthews, B. W. J. molec. Biol. 169, 757−769 (1983). | PubMed | ISI | ChemPort |
18. Steitz, T. A., Ohlendorf, D. H., McKay, D. B., Anderson, W. F. & Matthews, B. W. Proc. natn. Acad. Sci. U.S.A. 79, 3097−3100 (1982). | ChemPort |
19. Ohlendorf, D. H., Anderson, W. F. & Matthews, B. W. J. molec. Evol. 19, 109−114 (1983). | PubMed | ISI | ChemPort |
20. Pabo, C. O., Krovatin, W., Jeffrey, A. & Sauer, R. T. Nature 298, 441−443 (1982). | PubMed | ISI | ChemPort |
21. Wulff, D. L., Mahoney, M., Shatzman, A. & Rosenberg, M. Proc. natn. Acad. Sci. U.S.A. 81, 555−559 (1984). | ChemPort |
22. Bennett, G. N. & Yanofsky, C. J. molec. Biol. 121, 179−192 (1978). | PubMed | ISI | ChemPort |
23. Arnott, S. & Hukins, D. W. L. Biochem. Biophys. Res. Commun. 47, 1504−1509 (1972). | PubMed | ISI | ChemPort |
24. Calladine, C. R. J. molec. Biol. 161, 343−352 (1982). | PubMed | ISI | ChemPort |
25. Dickerson, R. E. J. molec. Biol. 166, 419−441 (1983). | PubMed | ISI | ChemPort |
26. Matthew, J. B. & Ohlendorf, D. H. J. biol. Chem. 260, 5860−5862 (1985). | PubMed | ISI | ChemPort |
27. Weber, I. T. & Steitz, T. A. Proc. natn. Acad. Sci. U.S.A. 81, 3973−3977 (1984). | ChemPort |
28. Lewis, M. et al. Cold Spring Harb. Symp. quant. Biol. 47, 435−440 (1983). | PubMed | ISI |
29. Ohlendorf, D. H., Anderson, W. F., Fisher, R. G., Takeda, Y. & Matthews, B. W. Nature 298, 718−723 (1982). | PubMed | ISI | ChemPort |
30. Hol, W. G. J., van Duijnen, P. T. & Berendsen, H. J. C. Nature 273, 443−446 (1978). | PubMed | ISI | ChemPort |
31. Oppenheim, D. S., Bennett, G. N. & Yanofsky, C. J. molec. Biol. 144, 133−142 (1980). | PubMed | ISI | ChemPort |



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