Nature 316, 696 - 700 (22 August 1985); doi:10.1038/316696a0

Amino-acid sequence of a Ca²⁺ + Mg²⁺ -dependent ATPase from rabbit muscle sarcoplasmic reticulum, deduced from its complementary DNA sequence

David H. MacLennan^*, Christopher J. Brandl^*, Bozena Korczak^* & N. Michael Green^†

^*Banting and Best Department of Medical Research, Charles H. Best Institute, University of Toronto, 112 College St, Toronto, Ontario, Canada M5G 1L6
^†National Institute for Medical Research, The Ridgeway, Mill Hill, London NW7 1AA, UK

We have cloned and sequenced complementary DNA encoding a Ca²⁺-ATPase of rabbit muscle sarcoplasmic reticulum. We propose a model of the protein which has 3 cytoplasmic domains joined to a set of 10 transmembrane helices by a narrow, penta-helical stalk. In this model. ATP bound to one cytoplasmic domain would phosphorylate an aspartate in an adjoining cytoplasmic domain, inducing translocation of Ca²⁺ from binding sites on the stalk.

References

1.	MacLennan, D. H. J. biol. Chem. 245, 4508−4518 (1970). | PubMed | ISI | ChemPort |
2.	MacLennan, D. H. & Reithmeier, R. A. F. in Membranes and Transport (ed. Martonosi, A. N.) Vol. 1 567−571 (Plenum, New York, 1982). | ChemPort |
3.	Thorley-Lawson, D. A. & Green, N. M. Eur. J. Biochem. 40, 403−413 (1973). | PubMed | ChemPort |
4.	Alien, G., Trinnaman, B. J. & Green, N. M. Biochem. J. 187, 591−616 (1980). | PubMed | ISI | ChemPort |
5.	DeFoor, P. H., Levitsky, D., Biryukova, T. & Fleischer, S. Archs Biochem. Biophys. 200, 196−205 (1980). | ChemPort |
6.	Damiani, E. et al. Biochem. J. 197, 245−248 (1981). | PubMed | ISI | ChemPort |
7.	Zubrzycka-Gaara, E., MacDonald, G., Phillips, L., Jorgensen, A. O. & MacLennan, D. H. J. Bioenerg. Biomembr. 16, 441−464 (1984). | PubMed |
8.	Klip, A., Reithmeier, R. A. F. & MacLennan, D. H. J. biol. Chem. 255, 6562−6568 (1980). | PubMed | ISI | ChemPort |
9.	Mitchinson, C., Wilderspin, A. F., Trinnaman, B. J. & Green, N. M. FEBS Lett. 146, 87−92 (1982). | Article | PubMed | ISI | ChemPort |
10.	Kozak, M. Nucleic Acids Res. 12, 857−872 (1984). | PubMed | ISI | ChemPort |
11.	Reithmeier, R. A. F., de Leon, S. & MacLennan, D. H. J. biol. Chem. 255, 11839−11846 (1980). | PubMed | ChemPort |
12.	Shull, G. E., Schwartz, A. & Lingrel, J. B. Nature 316, 691−695 (1985). | Article | PubMed | ISI | ChemPort |
13.	Proudfoot, N. J. & Brownlee, G. G. Nature 263, 211−214 (1976). | PubMed | ISI | ChemPort |
14.	McLauchlan, J., Gaffney, D., Whittan, J. L. & Clements, J. B. Nucleic Acids Res. 13, 1347−1369 (1985). | PubMed | ISI | ChemPort |
15.	Hayday, A. C. et al. Nature 307, 334−340 (1984). | PubMed | ISI | ChemPort |
16.	Hesse, J. E. et al. Proc. natn. Acad. Sci. U.S.A. 81, 4764−4750 (1984).
17.	Green, N. M. & Flanagan, M. T. Biochem. J. 153, 729−732 (1976). | PubMed | ISI | ChemPort |
18.	Kyte, J. & Doolittle, R. F. J. molec. Biol. 157, 105−132 (1983). | ISI |
19.	Taylor, W. R. & Thornton, J. M. J. molec. Biol. 173, 487−514 (1984). | PubMed | ISI | ChemPort |
20.	Shamoo, A. E., Ryan, T. E., Stewart, P. S. & MacLennan, D. H. J. biol. Chem. 251, 4147−4154 (1976). | PubMed | ISI | ChemPort |
21.	MacLennan, D. H. et al. Ann. N. Y. Acad. Sci. 358, 138−148 (1980). | PubMed | ChemPort |
22.	Pick, U. & Racker, E. Biochemistry 18, 108−113 (1979). | PubMed | ISI | ChemPort |
23.	Ludi, H. & Hasselbach, W. FEBS Lett. 167, 33−36 (1984). | Article | PubMed | ISI | ChemPort |
24.	Alien, G. & Green, N. M. FEBS Lett. 63, 188−192 (1976). | Article | PubMed | ISI | ChemPort |
25.	Scott, T. L. & Shamoo, A. E. J. membrane Biol. 64, 137−1444 (1982). | ISI | ChemPort |
26.	Jorgensen, P. L., Skriver, E., Hebert, H. & Maunsbach, A. B. Ann. N. Y. Acad. Sci. 402, 207−255 (1982). | PubMed | ISI | ChemPort |
27.	Richardson, J. S. Adv. Protein Chem. 34, 167−339 (1981). | PubMed | ChemPort |
28.	Bennett, W. S. & Huber, R. CRC Crit. Rev. Biochem. 15, 291−384 (1984). | PubMed | ISI | ChemPort |
29.	Sternberg, M. J. E. & Taylor, W. R. FEBS Lett. 175, 387−392 (1984). | Article | PubMed | ISI | ChemPort |
30.	Greaser, M. L., Cassens, R. G., Hoekstra, W. G. & Briskey, E. J. J. cell. Physiol. 74, 3750 (1969).
31.	Kretsinger, R. H. A. Rev. Biochem. 45, 239−266 (1976). | Article | ISI | ChemPort |
32.	MacLennan, D. H., Seeman, P., lies, G. H. & Yip, C. C. J. biol. Chem. 246, 2702−2710 (1971). | PubMed | ISI | ChemPort |
33.	Inesi, G. A. Rev. Physiol. 47, 573−601 (1985). | Article | ISI | ChemPort |
34.	Pick, U. & Karlish, S. J. D. Biochim. biophys. Acta 626, 255−261 (1980). | Article | PubMed | ISI | ChemPort |
35.	LeMaire, M., Møller, J. V. & Tardieu, A. J. molec. Biol. 150, 273−296 (1981). | PubMed | ISI | ChemPort |
36.	Okayama, H. & Berg, P. Molec. cell. Biol. 3, 280−289 (1983). | PubMed | ISI | ChemPort |
37.	MacLennan, D. H. & de Leon, S. Meth. Enzym. 96, 570−579 (1983). | Article | PubMed | ISI | ChemPort |
38.	Green, N. M. & Toms, E. J. Biochem. J. (in the press).
39.	Dente, L., Cesareni, G. & Cortese, R. Nucleic Acids Res. 11, 1645−1655 (1983). | PubMed | ISI | ChemPort |
40.	Sanger, F., Nicklen, S. & Coulson, A. R. Proc. natn. Acad. Sci. U.S.A. 74, 5463−5467 (1977). | ChemPort |
41.	Taylor, K., Dux, L. & Martonosi, A. J. molec. Biol. 174, 193−204 (1984). | PubMed | ISI | ChemPort |