Nature 316, 517 - 523 (08 August 1985); doi:10.1038/316517a0

The murine T-cell receptor uses a limited repertoire of expressed V gene segments

Richard K. Barth^*, Byung S. Kim^*†, Nancy C. Lan^*, Tim Hunkapiller^*, Nancy Sobieck^*, Astar Winoto^*, Howard Gershenfeld^‡, Craig Okada^‡, Dan Hansburg^§, Irving L. Weissman^‡ & Leroy Hood^*

^*Division of Biology, California Institute of Technology, Pasadena, California 91125, USA
^‡Department of Pathology, Stanford Medical School, Palo Alto, California 94304, USA
^§Department of Pathology, Fox Chase Cancer Center, Philadelphia, Pennsylvania 19111, USA
^†Permanent address: Department of Microbiology/Immunology, Northwestern University, Chicago, Illinois 60611, USA.

Only 10 different V gene segments were found when the sequences of 15 variable (V) genes of the mouse T-cell receptor were examined. From this analysis we calculate that the total number of expressed V gene segments may be 21 or fewer, which makes the expressed germline V repertoire much smaller than that of the immunoglobulin heavy-chain or light-chain genes. We suggest that chain somatic diversification is concentrated at the V-D-J junctions.

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