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Mismatch-specific post-meiotic segregation frequency in yeast suggests a heteroduplex recombination intermediate

Nature volume 315, pages 350–352 (1985)Cite this article

Abstract

Post-meiotic segregation of alleles, which is seen, for example, in the 5:3 distribution of alleles in the products of a single meiosis in fungi, has been thought to be due to the non-repair of heteroduplex regions formed during genetic recombination. In current models of genetic recombination, heteroduplex DNA is formed either as the primary intermediate generated by two interacting non-sister chromatids1 or as a short region flanking a double-stranded gap2. The frequency of post-meiotic segregation differs for different alleles, and this is presumed to reflect the varying efficiencies with which different types of mismatches in the heteroduplex are repaired. To gain some insight into this process, we have now determined the nucleotide sequences of various yeast alleles with different post-meiotic segregation frequencies and compared the mismatches predicted to occur in heteroduplexes of these alleles with wild-type DNA with those repaired with varying efficiency in bacterial systems. A striking correlation is observed, with the mismatches predicted for high post-meiotic segregation frequency alleles being similar to mismatches repaired with low efficiency in bacteria. These results support the view that post-meiotic segregation frequency reflects heteroduplex repair efficiency and the contention that meiotic gene conversion is the result of the successful repair of heteroduplex mismatches.

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Authors and Affiliations

  1. Department of Genetics, University of California, Berkeley, California, 94720, USA

    James H. White, Karin Lusnak & Seymour Fogel

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  1. James H. White
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  2. Karin Lusnak
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  3. Seymour Fogel
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White, J., Lusnak, K. & Fogel, S. Mismatch-specific post-meiotic segregation frequency in yeast suggests a heteroduplex recombination intermediate. Nature 315, 350–352 (1985). https://doi.org/10.1038/315350a0

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