Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

  • Article
  • Published:

Six unidentified reading frames of human mitochondrial DNA encode components of the respiratory-chain NADH dehydrogenase

Nature volume 314, pages 592–597 (1985)Cite this article

Abstract

The products of six unidentified reading frames of human mitochondrial DNA are precipitated from a mitochondrial lysate by antibodies against highly purified native beef heart NADH-ubiqutnone oxidoreductase (complex I). These products are enriched greatly in a human submitochondrial fraction enriched in NADH-Q1 and NADH-K3Fe(CN)6 oxidoreductase activities. We conclude that the six reading frames encode components of the respiratory-chain NADH dehydrogenase.

This is a preview of subscription content, access via your institution

Access options

Buy this article

  • Purchase on Springer Link
  • Instant access to full article PDF
Buy now

Prices may be subject to local taxes which are calculated during checkout

Similar content being viewed by others

References

  1. Anderson, S. et al. Nature 290, 457–465 (1981).

    Article  ADS  CAS  Google Scholar 

  2. Bibb, M. J., Van Etten, R. A., Wright, C. T., Walberg, M. W. & Clayton, D. A. Cell 26, 167–180 (1981).

    Article  CAS  Google Scholar 

  3. Anderson, S. et al. J. molec. Biol. 156, 683–717 (1982).

    Article  CAS  Google Scholar 

  4. Pepe, G. et al. Biochem. Int. 6, 553–563 (1983).

    CAS  PubMed  Google Scholar 

  5. Clary, D. O. & Wolstenholme, D. R. Nucleic Acids Res. 11, 6859–6872 (1983).

    Article  CAS  Google Scholar 

  6. Clary, D. O., Wahleithner, J. A. & Wolstenholme, D. R. Nucleic Acids Res. 12, 3747–3762 (1984).

    Article  CAS  Google Scholar 

  7. DeBruijn, M. H. L. Nature 304, 234–241 (1983).

    Article  ADS  CAS  Google Scholar 

  8. Davies, R. W. et al. in Mitochondrial Genes (eds Slonimski, P., Borst, P. & Attardi, G.) 405–410 (Cold Spring Harbor Laboratory, New York, 1982).

    Google Scholar 

  9. Scazzocchio, C., Brown, T. A., Waring, R. B., Ray, J. A. & Davies, R. W. in Mitochondria 1983. Nucleo-Mitochondrial Interactions (eds Schweyen, R. J., Wolf, K. & Kaudewitz, F.) 303–312 (de Gruyter, Berlin, 1983).

    Google Scholar 

  10. Hensgens, L. A. M. et al. Nucleic Acids Res. 12, 7327–7344 (1984).

    Article  CAS  Google Scholar 

  11. De La Cruz, V. F., Neckelmann, N. & Simpson, L. J. biol. Chem. 259, 15136–15147 (1984).

    CAS  PubMed  Google Scholar 

  12. Mariottini, P. et al. Cell 32, 1269–1277 (1983).

    Article  CAS  Google Scholar 

  13. Chomyn, A. et al. Proc. natn. Acad. Sci. U.S.A. 80, 5535–5539 (1983).

    Article  ADS  CAS  Google Scholar 

  14. Macreadie, I. G. et al. Nucleic Acids Res. 11, 4435–4451 (1983).

    Article  CAS  Google Scholar 

  15. Attardi, G. Int. Rev. Cytol. Suppl. 17 (in the press).

  16. Kyte, J. & Doolittle, R. F. J. molec. Biol. 157, 105–132 (1982).

    Article  CAS  Google Scholar 

  17. Hatefi, Y., Haavik, A. G. & Griffiths, D. E. J. biol. Chem. 237, 1676–1680 (1962).

    CAS  PubMed  Google Scholar 

  18. Hatefi, Y. & Stiggall, D. L. Enzymes 13, 175–297 (1976).

    Article  CAS  Google Scholar 

  19. Hare, J. F. & Hodges, R. Biochem. biophys. Res. Commun. 105, 1250–1256 (1982).

    Article  CAS  Google Scholar 

  20. Ragan, C. I. in Subcellular Biochemistry Vol. 7 (ed. Roodyn, D. B.) 267–307 (Plenum, New York, 1980).

    Book  Google Scholar 

  21. Hatefi, Y., Ragan, C. I. & Galante, Y. M. in The Enzymes of Biological Membranes Vol. 4 (ed. Martonosi. A.) 1–70 (Plenum, New York, 1984).

    Google Scholar 

  22. Hare, J. F., Ching, E. & Attardi, G. Biochemistry 19, 2023–2030 (1980).

    Article  CAS  Google Scholar 

  23. Ching, E. & Attardi, G. Biochemistry 21, 3188–3195 (1982).

    Article  CAS  Google Scholar 

  24. Heron, C., Smith, S. & Ragan, C. I. Biochem. J. 181, 435–443 (1979).

    Article  CAS  Google Scholar 

  25. Smith, S. & Ragan, C. I. Biochem. J. 185, 315–326 (1980).

    Article  CAS  Google Scholar 

  26. Smith, S., Cottingham, I. R. & Ragan, C. I. FEBS Lett. 110, 279–282 (1980).

    Article  CAS  Google Scholar 

  27. Storrie, B. & Attardi, G. J. molec. Biol. 71, 177–199 (1972).

    Article  CAS  Google Scholar 

  28. Hatefi, Y. & Stiggall, D. L. Meth. Enzym. 31, 5–10 (1978).

    Article  Google Scholar 

  29. Marzuki, S. et al. in Mitochondria 1983. Nucleo-Mitochondrial Interactions (eds Schweyen, R. J., Wolf, K. & Kaudewitz, F.) 535–549 (de Gruyter, Berlin, 1983).

    Google Scholar 

  30. Malczewski, R. M. & Whitfield, C. D. J. biol. Chem. 259, 11103–11113 (1984).

    CAS  PubMed  Google Scholar 

  31. Chu, E. H. Y., Sun, N. C. & Chang, C. C. Proc. natn. Acad. Sci. U.S.A. 69, 3459–3463 (1972).

    Article  ADS  CAS  Google Scholar 

  32. Malczewski, R. M. & Whitfield, C. D. J. biol. Chem. 257, 8137–8142 (1982).

    CAS  PubMed  Google Scholar 

  33. Ohnishi, T. Biochim. biophys. Acta 301, 105–128 (1973).

    Article  CAS  Google Scholar 

  34. Attardi, G., Chomyn, A. & Mariottini, P. in H+-ATP Synthase: Structure, Function, Regulation (eds Papa, S., Altendorg, K., Ernster, L. & Packer, L.) 25–40 (ICSU and Adriatica Editrice Bari).

  35. Galante, Y. M. & Hatefi, Y. Archs Biochem. Biophys. 192, 559–568 (1979).

    Article  CAS  Google Scholar 

  36. Hanstein, W. G., Davis, K. A., Ghalambor, M. A. & Hatefi, Y. Biochemistry 10, 2517–2524 (1971).

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Division of Biology, California Institute of Technology, Pasadena, California, 91125, USA

    Anne Chomyn, Paolo Mariottini & Giuseppe Attardi

  2. Department of Biochemistry, University of Southampton, Southampton, SO9 3TU, UK

    Michael W. J. Cleeter & C. Ian Ragan

  3. Division of Biochemistry, Department of Basic and Clinical Research, Scripps Clinic and Research Foundation, La Jolla, California, 92037, USA

    Akemi Matsuno-Yagi & Youssef Hatefi

  4. Department of Chemistry, University of California at San Diego, La Jolla, California, 92093, USA

    Russell F. Doolittle

Authors
  1. Anne Chomyn
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Paolo Mariottini
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Michael W. J. Cleeter
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. C. Ian Ragan
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Akemi Matsuno-Yagi
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Youssef Hatefi
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Russell F. Doolittle
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Giuseppe Attardi
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Chomyn, A., Mariottini, P., Cleeter, M. et al. Six unidentified reading frames of human mitochondrial DNA encode components of the respiratory-chain NADH dehydrogenase. Nature 314, 592–597 (1985). https://doi.org/10.1038/314592a0

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1038/314592a0

This article is cited by

Comments

By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.

Search

Advanced search

Quick links

Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing