Nature 312, 330 - 337 (22 November 1984); doi:10.1038/312330a0

Expression of active human factor VIII from recombinant DNA clones

William I. Wood, Daniel J. Capon, Christian C. Simonsen, Dan L. Eaton^*, Jane Gitschier, Bruce Keyt^*, Peter H. Seeburg, Douglas H. Smith, Philip Hollingshead, Karen L. Wion, Eric Delwart, Edward G. D. Tuddenham^†, Gordon A. Vehar^* & Richard M. Lawn

Departments of Molecular Biology and ^*Protein Biochemistry, Genentech, Inc., 460 Point San Bruno Boulevard, South San Francisco, California 94080, USA
^†Haemophilia Centre, Royal Free Hospital School of Medicine, London WC1N 1BP, UK

DNA clones encoding the complete 2,351 amino acid sequence for human factor VIII have been isolated and used to produce biologically active factor VIII in cultured mammalian cells. The recombinant protein corrects the clotting time of plasma from haemophiliacs and has many of the biochemical and immunological characteristics of serum-derived factor VIII.

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