Nature Publishing Group, publisher of Nature, and other science journals and reference works
Nature
my account e-alerts subscribe register
   
Tuesday 27 June 2017
Journal Home
Current Issue
AOP
Archive
Download PDF
References
Export citation
Export references
Send to a friend
More articles like this

Article
Nature 312, 121 - 127 (08 November 1984); doi:10.1038/312121a0

Primary structure of Electrophorus electricus sodium channel deduced from cDNA sequence

Masaharu Noda, Shin Shimizu, Tsutomu Tanabe, Toshiyuki Takai, Toshiaki Kayano, Takayuki Ikeda, Hideo Takahashi, Hitoshi Nakayama*, Yuichi Kanaoka*, Naoto Minamino, Kenji Kangawa, Hisayuki Matsuo, Michael A. Raftery, Tadaaki Hirose§, Seiichi Inayama§, Hidenori Hayashida, Takashi Miyata & Shosaku Numa         

Department of Medical Chemistry, Kyoto University Faculty of Medicine, Kyoto 606, Japan
*Faculty of Pharmaceutical Sciences, Hokkaido University, Sapporo 060, Japan
Department of Biochemistry, Miyazaki Medical College, Miyazaki 889-16, Japan
Division of Chemistry and Chemical Engineering, California Institute of Technology, Pasadena, California 91125, USA
§Pharmaceutical Institute, Keio University School of Medicine, Tokyo 160, Japan
Department of Biology, Kyushu University Faculty of Science, Fukuoka 812, Japan

Cloning and sequence analysis of cDNA for the Electrophorus electricus electroplax sodium channel indicate that this protein, consisting of 1,820 amino acid residues, exhibits four repeated homology units, which are presumably oriented in a pseudosymmetric fashion across the membrane. Each homology unit contains a unique segment with clustered positively charged residues, which may be involved in the gating structure, possibly in conjunction with negatively charged residues clustered elsewhere.

------------------

References

1. Agnew, W. S. A. Rev. Physiol. 46, 517–530 (1984). | Article | ISI | ChemPort |
2. Catterall, W. A. Science 223, 653–661 (1984). | PubMed | ISI | ChemPort |
3. Hodgkin, A. L. & Huxley, A. F. J. Physiol., Lond. 117, 500–544 (1952). | PubMed | ISI | ChemPort |
4. Catterall, W. A. A. Rev. Pharmac. Tox. 20, 15–43 (1980). | Article | ISI | ChemPort |
5. Agnew, W. S., Levinson, S. R., Brabson, J. S. & Raftery, M. A. Proc. natn. Acad. Sci. U.S.A. 75, 2606–2610 (1978). | ChemPort |
6. Nakayama, H., Withy, R. M. & Raftery, M. A. Proc. natn. Acad. Sci. U.S.A. 79, 7575–7579 (1982). | ChemPort |
7. Miller, J. A., Agnew, W. S. & Levinson, S. R. Biochemistry 22, 462–470 (1983). | PubMed | ISI | ChemPort |
8. Hartshorne, R. P. & Catterall, W. A. Proc. natn. Acad. Sci. U.S.A. 78, 4620–4624 (1981). | ChemPort |
9. Barchi, R. L. J. Neurochem. 40, 1377–1385 (1983). | PubMed | ISI | ChemPort |
10. Lombet, A. & Lazdunski, M. Eur. J. Biochem. 141, 651–660 (1984). | PubMed | ISI | ChemPort |
11. Weigele, J. B. & Barchi, R. L. Proc. natn. Acad. Sci. U.S.A. 79, 3651–3655 (1982). | ChemPort |
12. Tamkun, M. M., Talvenheimo, J. A. & Catterall, W. A. J. biol. Chem. 259, 1676–1688 (1984). | PubMed | ISI | ChemPort |
13. Rosenberg, R. L., Tomiko, S. A. & Agnew, W. S. Proc. natn. Acad. Sci. U.S.A. 81, 1239–1243 (1984). | ChemPort |
14. Hanke, W., Boheim, G., Barhanin, J., Pauron, D. & Lazdunski, M. EMBO J. 3, 509–515 (1984). | PubMed | ISI | ChemPort |
15. Hewick, R. M., Hunkapiller, M. W., Hood, L. E. & Dreyer, W. J. J. biol. Chem. 256, 7990–7997 (1981). | PubMed | ISI | ChemPort |
16. Vieira, J. & Messing, J. Gene 19, 259–268 (1982). | Article | PubMed | ISI | ChemPort |
17. Helfman, D. M., Feramisco, J. R., Fiddes, J. C., Thomas, G. P. & Hughes, S. H. Proc. natn. Acad. Sci. U.S.A. 80, 31–35 (1983). | ChemPort |
18. Southern, E. J. molec. Biol. 98, 503–517 (1975). | PubMed | ISI | ChemPort |
19. Maxam, A. M. & Gilbert, W. Meth. Enzym. 65, 499–560 (1980). | Article | PubMed | ChemPort |
20. Thomas, P. S. Proc. natn. Acad. Sci. U.S.A. 77, 5201–5205 (1980). | ChemPort |
21. Okayama, H. & Berg, P. Molec. cell. Biol. 2, 161–170 (1982). | PubMed | ISI | ChemPort |
22. Hanahan, D. & Meselson, M. Gene 10, 63–67 (1980). | Article | PubMed | ISI | ChemPort |
23. Kozak, M. Nucleic Acids Res. 9, 5233–5252 (1981). | PubMed | ISI | ChemPort |
24. Proudfoot, N. J. & Brownlee, G. G. Nature 263, 211–214 (1976). | PubMed | ISI | ChemPort |
25. Hubbard, S. C. & Ivatt, R. J. A. Rev. Biochem. 50, 555–583 (1981). | Article | ISI | ChemPort |
26. Bause, E. Biochem. J. 209, 331–336 (1983). | PubMed | ISI | ChemPort |
27. Toh, H., Hayashida, H. & Miyata, T. Nature 305, 827–829 (1983). | PubMed | ISI | ChemPort |
28. Chou, P. Y. & Fasman, G. D. A. Rev. Biochem. 47, 251–276 (1978). | Article | ISI | ChemPort |
29. Kyte, J. & Doolittle, R. F. J. molec. Biol. 157, 105–132 (1982). | PubMed | ISI | ChemPort |
30. Capaldi, R. A. & Vanderkooi, G. Proc. natn. Acad. Sci. U.S.A. 69, 930–932 (1972). | ChemPort |
31. Engelman, D. M. & Steitz, T. A. Cell 23, 411–422 (1981). | Article | PubMed | ISI | ChemPort |
32. Rice, C. M., Bell, J. R., Hunkapiller, M. W., Strauss, E. G. & Strauss, J. H. J. molec. Biol. 154, 355–378 (1982). | PubMed | ISI | ChemPort |
33. Hille, B. J. gen. Physiol. 58, 599–619 (1971). | Article | PubMed | ISI | ChemPort |
34. Hille, B. J. gen. Physiol. 59, 637–658 (1972). | Article | PubMed | ISI | ChemPort |
35. Hille, B. in Membranes: A Series of Advances Vol. 3 (ed. Eisenman, G.) 255–323 (Dekker, New York, 1975). | ChemPort |
36. Kreil, G. A. Rev. Biochem. 50, 317–348 (1981). | Article | ISI | ChemPort |
37. Blobel, G. & Dobberstein, B. J. Cell Biol. 67, 852–862 (1975). | Article | PubMed | ISI | ChemPort |
38. Braell, W. A. & Lodish, H. F. Cell 28, 23–31 (1982). | Article | PubMed | ISI | ChemPort |
39. Anderson, D. J., Mostov, K. E. & Blobel, G. Proc. natn. Acad. Sci. U.S.A. 80, 7249–7253 (1983). | ChemPort |
40. Claesson, L., Larhammar, D., Rask, L. & Paterson, P. A. Proc. natn. Acad. Sci. U.S.A. 80, 7395–7399 (1983). | ChemPort |
41. Chin, D. J. et al. Nature 308, 613–617 (1984). | PubMed | ISI | ChemPort |
42. Armstrong, C. M. & Bezanilla, F. Nature 242, 459–461 (1973). | PubMed | ISI | ChemPort |
43. Armstrong, C. M. Q. Rev. Biophys. 7, 179–210 (1975). | ISI |
44. Armstrong, C. M. Physiol. Rev. 61, 644–683 (1981). | PubMed | ISI | ChemPort |
45. Laemmli, U. K. Nature 227, 680–685 (1970). | PubMed | ISI | ChemPort |
46. Sutcliffe, J. G. Nucleic Acids Res. 5, 2721–2728 (1978). | PubMed | ISI | ChemPort |
47. Reddy, V. B. et al. Science 200, 494–502 (1978). | PubMed | ISI | ChemPort |
48. Chirgwin, J. M., Przybyla, A. E., MacDonald, R. J. & Rutter, W. J. Biochemistry 18, 5294–5299 (1979). | PubMed | ISI | ChemPort |
49. Aviv, H. & Leder, P. Proc. natn. Acad. Sci. U.S.A. 69, 1408–1412 (1972). | ChemPort |
50. Hanahan, D. J. molec. Biol. 166, 557–580 (1983). | PubMed | ISI | ChemPort |
51. Moore, H.-P. H., Fritz, L. C., Raftery, M. A. & Brockes, J. P. Proc. natn. Acad. Sci. U.S.A. 79, 1673–1677 (1982). | ChemPort |
52. Ito, H., Ike, Y., Ikuta, S. & Itakura, K. Nucleic Acids Res. 10, 1755–1769 (1982). | PubMed | ISI | ChemPort |
53. Boyer, H. W. & Roulland-Dussoix, D. J. molec. Biol. 41, 459–472 (1969). | PubMed | ISI | ChemPort |
54. Wahl, G. M., Padgett, R. A. & Stark, G. R. J. biol. Chem. 254, 8679–8689 (1979). | PubMed | ISI | ChemPort |
55. Weinstock, R., Sweet, R., Weiss, M., Cedar, H. & Axel, R. Proc. natn. Acad. Sci. U.S.A. 75, 1299–1303 (1978). | ChemPort |
56. Noda, M. et al. Nature 295, 202–206 (1982). | PubMed | ISI | ChemPort |
57. McMaster, G. K. & Carmichael, G. G. Proc. natn. Acad. Sci. U.S.A. 74, 4835–4838 (1977). | ChemPort |
58. Dayhoff, M. O., Schwartz, R. M. & Orcutt, B. C. in Atlas of Protein Sequence and Structure Vol. 5, Suppl. 3 (ed. Dayhoff, M. O.) 345–352 (National Biomedical Research Foundation, Silver Spring, Maryland, 1978).



© 1984 Nature Publishing Group
Privacy Policy