Nature 310, 789 - 792 (30 August 1984); doi:10.1038/310789a0

Immune sera recognize on erythrocytes a Plasmodium falciparum antigen composed of repeated amino acid sequences

Ross L. Coppel, Alan F. Cowman, Robin F. Anders, Albert E. Bianco, Robert B. Saint, Klaus R. Lingelbach, David J. Kemp & Graham V. Brown

The Walter and Eliza Hall Institute of Medical Research, Post Office, Royal Melbourne Hospital, Victoria 3050, Australia

Protective immune responses against the asexual stages of the human malaria parasite, Plasmodium falciparum, are most probably directed against exposed antigenic determinants on the surface of the free merozoite¹⁻³ or the infected red blood cell⁴, and therefore antigens in these locations are candidates for testing as components of a defined molecular vaccine. To facilitate the search for such antigens, we recently developed a method for the expression of P. falciparum proteins in Escherichia coli as fused polypeptides⁵. Many clones producing antigens were detected by screening with immune human sera^6,7. We show here that antibodies against the fused polypeptide expressed by one such clone react with a P. falciparum protein that is synthesized late in schizogony and is later present on the surface of the ring-infected erythrocyte. The protein is composed of repeating subunits of 8, 4 and 3 amino acids and is present in all isolates of P. falciparum examined.

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