Nature 310, 207 - 211 (19 July 1984); doi:10.1038/310207a0

DNA sequence and expression of the B95-8 Epstein—Barr virus genome

R. Baer^*, A. T. Bankier, M. D. Biggin, P. L. Deininger^*, P. J. Farrell^†, T. J. Gibson, G. Hatfull, G. S. Hudson, S. C. Satchwell, C. Séguin^*, P. S. Tuffnell^† & B. G. Barrell

MRC Laboratory of Molecular Biology and ^†Ludwig Institute for Cancer Research, MRC Centre, Hills Road, Cambridge CB2 2QH, UK
^*Present addresses: Memorial Sloan-Kettering Cancer Center, 1275 York Avenue, New York, New York 10021, USA (R.B.); LSU Medical Center, 1901 Perdido St, New Orleans, Louisiana 70112, USA (P.L.D.); Cellular Regulation Section, Laboratory of Biochemistry, NCI, National Institutes of Health, Bethesda, Maryland 20205, USA (C.S.).

The complete (172,282 base pairs) nucleotide sequence of the B95-8 strain of Epstein−Barr virus has been established using the dideoxynucleotide/M13 sequencing procedure. Many RNA polymerase II promoters have been mapped and the mRNAs from these promoters have been assigned to the latent or early/late productive virus cycles. Likely protein-coding regions have been identified and three of these have been shown to encode a ribonucleotide reductase, a DNA polymerase and two surface glycoproteins.

References

1.	Epstein, M. A., Achong, B. G. & Barr, Y. M. Lancet i, 702−703 (1964). | Article |
2.	Roizman, B. (ed.) The Herpesviruses, The Viruses Vol. 1 (Plenum, New York, 1982).
3.	Epstein, M. A. & Achong, B. G. (eds) The Epstein−Barr Virus (Springer, Berlin, 1979).
4.	Henle, W., Diehl, V., Kohn, G. , zur Hausen, H. & Henle, G. Science 157, 1064−1065 (1967). | PubMed | ISI | ChemPort |
5.	Pope, J. H., Horne, M. K. & Scott, W. Int. J. Cancer 3, 857−866 (1968). | PubMed | ISI | ChemPort |
6.	Jondal, M. & Klein, G. J. exp. Med. 138, 1365−1378 (1973). | Article | PubMed | ISI | ChemPort |
7.	Morgan, D. G., Niederman, J. C., Miller, G., Smith, H. W. & Dowaliby, J. M. Lancei ii, 1154−1157 (1979). | Article |
8.	Lemon, S. M., Hutt, L. M., Shaw, J. E., Li, J. L. H. & Pagano, J. S. Nature 268, 268−270 (1977). | PubMed | ISI | ChemPort |
9.	Miller, G., Shope, T., Lisco, H., Stitt, D. & Lipman, M. Proc. natn. Acad. Sci. U.S.A. 69, 383−387 (1972). | ChemPort |
10.	zur Hausen, H., Hecker, E., O'Neill, F. J. & Freese, U. K. Nature 272, 373−375 (1978). | PubMed | ChemPort |
11.	Pritchett, R. F., Hayward, S. D. & Kieff, E. J. Virol. 15, 556−569 (1975). | PubMed | ISI | ChemPort |
12.	Hayward, S. D. & Kieff, E. J Virol. 23, 421−429 (1977). | PubMed | ISI | ChemPort |
13.	Lindahl, T. et al. J. molec. Biol. 102, 511−530 (1976). | PubMed | ISI | ChemPort |
14.	Dambaugh, T. et al. Proc. natn. Acad. Sci. U.S.A. 77, 2999−3003 (1980). | ChemPort |
15.	Given, D., Yee, D., Griem, K. & Kieff, E. J. Virol. 30, 852−862 (1979). | PubMed | ISI | ChemPort |
16.	Kintner, C. R. & Sugden, B. Cell 17, 661−671 (1979). | Article | PubMed | ISI | ChemPort |
17.	Given, D. & Kieff, E. J. Virol. 31, 315−324 (1979). | PubMed | ISI | ChemPort |
18.	Rymo, L. & Forsblom, S. Nucleic Acids Res. 5, 1387−1402 (1978). | PubMed | ISI | ChemPort |
19.	Hayward, S. D., Nogee, L. & Hayward, G. S. J. Virol. 33, 507−521 (1980). | PubMed | ISI | ChemPort |
20.	Skare, J. & Strominger, J. L. Proc. natn. Acad. Sci. U.S.A. 77, 3860−3864, 7510 (1980). | ChemPort |
21.	Arrand, J. R. et al. Nucleic Acids Res. 9, 2999−3014 (1981). | PubMed | ISI | ChemPort |
22.	Hummel, M. & Kieff, E. J. Virol. 43, 262−272 (1982). | PubMed | ISI | ChemPort |
23.	Weigel, R. & Miller, G. Virology 125, 287−298 (1983). | Article | PubMed | ISI | ChemPort |
24.	Hummel, M. & Kieff, E. Proc. natn. Acad. Sci. U.S.A. 79, 5698−5702 (1982). | ChemPort |
25.	Seibl, R. & Wolf, H. Virology (submitted).
26.	Cohen, L. K., Spech, S. H., Roberts, B. E. & Strominger, J. L. Proc. natn. Acad. Sci. U.S.A. (in the press).
27.	Deininger, P. Analyt. Biochem. 129, 216−223 (1983). | PubMed | ISI | ChemPort |
28.	Messing, J. & Vieira, J. Gene 19, 269−276 (1982). | Article | PubMed | ISI | ChemPort |
29.	Sanger, F., Nicklen, S. & Coulson, A. R. Proc. natn. Acad. Sci. U.S.A. 74, 5463−5467 (1977). | ChemPort |
30.	Staden, R. Nucleic Acids Res. 10, 4731−4751 (1982); 12, 499−503 (1984). | PubMed | ISI | ChemPort |
31.	Bankier, A. T. & Barrell, B. G. Techniques in the Life Sciences Vol. B5 (Elsevier, Ireland, 1983).
32.	Bankier, A. T. et al. Molec. Biol. Med. 1, 425−445 (1983). | PubMed | ChemPort |
33.	Bankier, A. T., Deininger, P. L., Farrell, P. J. & Barrell, B. G. Molec. Biol. Med. 1, 21−45 (1983). | PubMed | ChemPort |
34.	Seguin, C. S., Farrell, P. J. & Barrell, B. G. Molec. Biol. Med. 1, 369−392 (1983). | PubMed | ChemPort |
35.	Biggin, M., Farrell, P. J. & Barrell, B. G. EMBO J. 3, 1083−1090 (1984). | PubMed | ISI | ChemPort |
36.	Gibson, T., Stockwell, P., Ginsberg, M. & Barrell, B. G. Nucleic Acids Res. (in the press).
37.	Farrell, P. J., Deininger, P. L., Bankier, A. T. & Barrell, B. G. Proc. natn. Acad. Sci. U.S.A. 80, 1565−1569 (1983). | ChemPort |
38.	Farrell, P. J., Bankier, A., Seguin, C., Deininger, P. & Barrell, B. G. EMBO J. 2, 1331−1338 (1983). | PubMed | ISI | ChemPort |
39.	Jeang, K. & Hayward, S. D. J. Virol. 48, 135−148 (1983). | PubMed | ISI | ChemPort |
40.	Jones, M., Foster, L., Sheedy, T. & Griffin, B. E. EMBO J. 3, 813−821 (1984). | PubMed | ISI | ChemPort |
41.	Hayward, S. D., Lazarowitz, S. G. & Hayward, G. S. J. Virol. 43, 201−212 (1982). | PubMed | ISI | ChemPort |
42.	Jones, M. D. & Griffin, B. E. Nucleic Acids Res. 11, 3919−3937 (1983). | PubMed | ISI | ChemPort |
43.	Cheung, A. & Kieff, E. J. Virol. 44, 286−294 (1982). | PubMed | ISI | ChemPort |
44.	Hennessy, K. & Kieff, E. Proc. natn. Acad. Sci. U.S.A. 80, 5665−5669 (1983). | ChemPort |
45.	Staden, R. Nucleic Acids Res. 12, 521−538 (1984). | PubMed | ISI | ChemPort |
46.	Raab-Traub, N., Pritchett, R. & Kieff, E. J. Virol. 27, 388−398 (1978). | PubMed | ChemPort |
47.	Delius, H. & Bornkamm, G. W. J. Virol. 27, 81−89 (1978). | PubMed | ISI | ChemPort |
48.	Arrand, J. R. & Rymo, L. J. Virol. 41, 376−379 (1982). | PubMed | ISI | ChemPort |
49.	Rosa, M. D., Gottlieb, E., Lerner, M. R. & Steitz, J. A. Molec. cell. Biol. 1, 785−796 (1981). | PubMed | ISI | ChemPort |
50.	Bhat, R. A. & Thimmappaya, B. Proc. natn. Acad. Sci. U.S.A. 80, 4789−4793 (1983). | ChemPort |
51.	Yates, J., Warren, N., Reisman, D. & Sugden, B. Proc. natn. Acad. Sci. U.S.A. (in the press).
52.	Manley, J. L., Fire, A., Cano, A., Sharp, P. A. & Gefter, M. Proc. natn. Acad. Sci. U.S.A. 77, 3855−3859 (1980). | ChemPort |
53.	Corden, J. et al. Science 209, 1406−1414 (1980). | PubMed | ISI | ChemPort |
54.	Summers, W. C. &Klein, G. J. Virol. 18, 151−155 (1976). | PubMed | ISI | ChemPort |
55.	Herz, C. & Roizman, B. Cell 33, 145−151 (1983). | Article | PubMed | ISI | ChemPort |
56.	Kieff, E. et al. J. infect. Dis. 146, 506−517 (1982). | PubMed | ISI | ChemPort |
57.	Rymo, L. J. Virol. 32, 8−18 (1979). | PubMed | ISI | ChemPort |
58.	Van Santen, V., Cheung, A., Hummel, M. & Kieff, E. J. Virol. 46, 424−433 (1983). | PubMed | ChemPort |
59.	Heller, M. , van Santen, V. & Kieff, E. J. Virol. 44, 311−320 (1982). | PubMed | ISI | ChemPort |
60.	Fischer, D. K. et al. Proc. natn. Acad. Sci. U.S.A. 81, 43−47 (1984). | ChemPort |
61.	Hennessy, K., Heller, M. , van Santen, V. & Kieff, E. Science 220, 1396−1398 (1983). | PubMed | ISI | ChemPort |
62.	Grogan, E. A. et al. Proc. natn. Acad. Sci. U.S.A. 80, 7650−7653 (1983). | ChemPort |
63.	Swain, M. A. & Galloway, D. A. J. Virol. 46, 1045−1050 (1983). | PubMed | ISI | ChemPort |
64.	Walker, J. E., Saraste, M., Runswick, M. J. & Gay, N. J. EMBO J. 1, 945−951 (1982). | PubMed | ISI | ChemPort |
65.	Hofmann, G. J., Lazarowitz, S. G. & Hayward, S. D. Proc. natn. Acad. Sci. U.S.A. 77, 2979−2983 (1980).
66.	Thorley-Lawson, D. A. & Poodry, C. A. J. Virol. 43, 730−736 (1982). | PubMed | ChemPort |
67.	Hummel, M., Thorley-Lawson, D. A. & Kieff, E. J. Virol. 49, 413−417 (1984). | PubMed | ISI | ChemPort |
68.	Klein, G. Cell 32, 311−315 (1983). | Article | PubMed | ISI | ChemPort |
69.	Griffin, B. E. & Karran, L. Nature 309, 78−82 (1984). | Article | PubMed | ISI | ChemPort |