Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

  • Article
  • Published:

Light-inducible and chloroplast-associated expression of a chimaeric gene introduced into Nicotiana tabacum using a Ti plasmid vector

Nature volume 310, pages 115–120 (1984)Cite this article

Abstract

A chimaeric gene, consisting of the 5′-flanking region of a member of the Pisum sativum gene family encoding ribulose 1,5-bisphosphate carboxylase linked to the coding region of a bacterial chloramphenicol acetyltransferase gene, has been introduced into the genome of the plant Nicotiana tabacum using a Ti plasmid of Agrobacterium tumefaciens. The expression of the chimaeric gene is light-inducible in chloroplast-containing transformed tissue.

This is a preview of subscription content, access via your institution

Access options

Buy this article

  • Purchase on Springer Link
  • Instant access to full article PDF
Buy now

Prices may be subject to local taxes which are calculated during checkout

Similar content being viewed by others

References

  1. Herrera-Estrella, L., Depicker, A., Van Montagu, M. & Schell, J. Nature 303, 209–213 (1983).

    Article  ADS  CAS  Google Scholar 

  2. Herrera-Estrella, L. et al. EMBO J. 2, 987–995 (1983).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  3. Bevan, M. W., Flavell, R. B. & Chilton, M.-D. Nature 304, 184–187 (1983).

    Article  ADS  CAS  Google Scholar 

  4. Fraley, R. T. et al. Proc. natn. Acad. Sci. U.S.A. 80, 4803–4807 (1983).

    Article  ADS  CAS  Google Scholar 

  5. Zambryski, P. et al. EMBO J. 2, 2143–2150 (1983).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  6. Dobberstein, B., Blobel, G. & Chua, N.-H. Proc. natn. Acad. Sci. U.S.A. 74, 1082–1085 (1977).

    Article  ADS  CAS  Google Scholar 

  7. Cashmore, A. R., Broadhurst, M. K. & Gray, R. E. Proc. natn. Acad. Sci. U.S.A. 75, 655–659 (1978).

    Article  ADS  CAS  Google Scholar 

  8. Highfield, P. E. & Ellis, R. J. Nature 271, 420–424 (1978).

    Article  ADS  CAS  Google Scholar 

  9. Chua, N. H. & Schmidt, G. W. Proc. natn. Acad. Sci. U.S.A. 75, 6110–6114 (1978).

    Article  ADS  CAS  Google Scholar 

  10. Cashmore, A. R. in Genetic Engineering of Plants-An Agricultural Perspective (eds Kosuge, T., Meredith, C. P. & Hollaender, A.) 29–38 (Plenum, New York, 1983).

    Book  Google Scholar 

  11. Berry-Lowe, S. L., McKnight, T. D., Shah, D. M. & Meagher, R. B. J. molec. appl. Genet. 1, 483–498 (1982).

    CAS  Google Scholar 

  12. Coruzzi, G., Broglie, R., Cashmore, A. R. & Chua, N.-H. J. biol. Chem. 258, 1399–G402 (1983).

    CAS  PubMed  Google Scholar 

  13. Broglie, R., Coruzzi, G., Lamppa, G., Keith, B. & Chua, N.-H. Bio/technology 1, 55–61 (1983).

    Google Scholar 

  14. Dunsmuir, P., Smith, S. & Bedbrook, J. Nucleic Acids Res. 11, 4177–4183 (1983).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  15. Bedbrook, J. R., Smith, S. M. & Ellis, R. J. Nature 287, 692–697 (1980).

    Article  ADS  CAS  Google Scholar 

  16. Smith, S. M. & Ellis, R. J. J. molec. appl. Genet. 1, 127–137 (1981).

    CAS  Google Scholar 

  17. Gallagher, T. F. & Ellis, R. J. EMBO J. 1, 1493–1498 (1982).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  18. Tobin, E. M. Pl. molec. Biol. 1, 35–51 (1981).

    Article  CAS  Google Scholar 

  19. Thompson, W. F., Everett, M., Polans, N. O., Jorgensen, R. A. & Palmer, J. D. Planta 158, 487–500 (1983).

    Article  CAS  PubMed  Google Scholar 

  20. Link, G., Coen, D. M. & Bogorad, L. Cell 15, 725–731 (1978).

    Article  CAS  PubMed  Google Scholar 

  21. Broglie, R., Coruzzi, G. & Chua, N.-H. in Chloroplast Biogenesis (Cambridge University Press, in the press)

  22. Shinzaki, K., Sasaki, Y., Sakibama, T. & Kamikubo, T. FEBS Lett. 144, 73–76 (1982).

    Article  Google Scholar 

  23. Cashmore, A. Cell 17, 383–388 (1979).

    Article  CAS  PubMed  Google Scholar 

  24. Brinster, R. L., Chen, H. Y., Warren, R., Sarthy, A. & Palmiter, R. D. Nature 296, 39–42 (1982).

    Article  ADS  CAS  PubMed  Google Scholar 

  25. Parker, M. Nature 304, 687–688 (1983).

    Article  ADS  CAS  PubMed  Google Scholar 

  26. Gorman, C. M., Mofft, L. F. & Howard, B. H. Molec. cell. Biol. 2, 1044–1051 (1982).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  27. Van Haute, E. et al. EMBO J. 2, 411–418 (1983).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  28. Southern, E. M. J. molec. Biol. 98, 503–518 (1975).

    Article  CAS  PubMed  Google Scholar 

  29. Depicker, A. et al. Plasmid 3, 193–211 (1980).

    Article  CAS  PubMed  Google Scholar 

  30. Vernet, T. et al. Eur. J. Biochem. 126, 489–494 (1982).

    Article  CAS  PubMed  Google Scholar 

  31. Rao, R. N. & Rogers, S. G. Gene 7, 79–82 (1979).

    Article  CAS  PubMed  Google Scholar 

  32. Sanger, F., Nicklen, S. & Coulsen, A. Proc. natn. Acad. Sci. U.S.A. 74, 5463–5467 (1977).

    Article  ADS  CAS  Google Scholar 

  33. De Greve, H. et al. J. molec. appl. Genet. 1, 499–512 (1982).

    CAS  Google Scholar 

  34. Thomas, P. Proc. natn. Acad. Sci. U.S.A. 77, 5201–5205 (1980).

    Article  ADS  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Laboratorium voor Genetica, Rijksuniversiteit Gent, Gent, B-9000, Belgium

    L. Herrera-Estrella, G. Van den Broeck, R. Maenhaut, M. Van Montagu & J. Schell

  2. Laboratory of Cell Biology, The Rockefeller University, New York, New York, 10021, USA

    M. Timko & A. Cashmore

Authors
  1. L. Herrera-Estrella
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. G. Van den Broeck
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. R. Maenhaut
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. M. Van Montagu
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. J. Schell
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. M. Timko
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. A. Cashmore
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Herrera-Estrella, L., Van den Broeck, G., Maenhaut, R. et al. Light-inducible and chloroplast-associated expression of a chimaeric gene introduced into Nicotiana tabacum using a Ti plasmid vector. Nature 310, 115–120 (1984). https://doi.org/10.1038/310115a0

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1038/310115a0

This article is cited by

Comments

By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.

Search

Advanced search

Quick links

Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing