Abstract
A series of automated instruments that use state-of-the-art chemical methods has been developed for high-sensitivity protein sequencing, DNA synthesis and peptide synthesis. These intruments have been integrated into a centralized microchemical facility in order to promote their use for the study of a variety of biologically interesting problems. This facility has as one of its major functions the development of new chemistries and instrumentation for the structural analysis and synthesis of genes and proteins.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Edman, P. Acta chem. scand. 10, 761–768 (1956).
Edman, P. & Begg, G. Eur. J. Biochem. 1, 80–91 (1967).
Hewick, R. M., Hunkapiller, M. W., Hood, L. E. & Dreyer, W. J. J. biol. Chem. 256, 7990–7997 (1981).
Hunkapiller, M. W. & Hood, L. E. Science 219, 650–659 (1983).
Tarr, G. E., Beecker, J. F., Bell, M. & McKean, D. J. Analyt. Biochem. 84, 622–627 (1978).
Bennett, J. P. J., Hudson, A. M., McMartin, C. & Purdon, G. E. Biochem. J. 168, 9–13 (1977).
Lewis, R. V., Fallon, A., Stein, S., Gibson, K. D. & Udenfriend, S. Analyt. Biochem. 104, 153–159 (1980).
Tempst, P., Hunkapiller, M. W. & Hood, L. E. Analyt. Biochem. 137, 188–195 (1984).
Hunkapiller, M. W., Lujan, E., Ostrander, F. & Hood, L. E. Meth. Enzym. 91, 227–236 (1983).
L'Italien, J. J. & Kent, S. B. H. 2nd int. Symp. on HPLC of Proteins, Peptides, and Polynucleotides, Baltimore, Maryland, December 1982, Abstr. 600; J. Chromatogr. 283, 149–156 (1984).
Giffin, C. E., Boettger, H. G. & Norris, D. D. Int. J. Mass Spectrom. Ion Phys. 15, 437–449 (1974).
Dreyer, W. J. et al. Clin. Chem. 20, 998–1002 (1974).
Boettger, H. G., Giffin, C. E. & Norris, D. D. in Monograph on Image Detectors in Chemistry (ed. Talmi, Y.) 291–318 (American Chemical Society, 1979).
Khorana, H.G. et al. J. molec. Biol. 72, 209–217 (1972).
Matteucci, M. D. & Caruthers, M. H. J. Am. chem. Soc. 103, 3185–3191 (1981).
Beaucage, S. L. & Caruthers, M. H. Tetrahedron Lett. 22, 1859–1862 (1981).
Horvath, S. J., Firca, J., Hood, L., Hunkapiller, T. & Hunkapiller, M. W. J. J. biol. Chem. (submitted).
Yajima, H. & Fugii, N. in Chemical Synthesis and Sequencing of Peptides and Proteins (eds Liu, T. Y., Schechter, A. M., Heinrikson, R. L. & Condliffe, P. G.) 21–39 (Elsevier, Amsterdam, 1981).
Barany, G. & Merrifield, R. B. in The Peptides Vol. 2 (eds Gross, E. & Meienhofer, J.) 1–284 (Academic, New York, 1980).
Kent, S. B. H., Mitchell, A. R., Engelhard, M. & Merrifield, R. B. Proc. natn. Acad. Sci. U.S.A. 76, 2180–2184 (1979).
Kent, S. B. H. in Biomedical Polymers (eds Goldberg, E. P. & Nakajima, A.) 213–242 (Academic, New York, 1980).
Sarin, V. K., Kent, S. B. H. & Merrifield, R. B. J. Am. chem. Soc. 102, 5463–5470 (1980).
Kent, S. B. H. & Merrifield, R. B. in Peptides 1980, Proc. 16th European Peptide Symp. (ed. Brunfeldt, K.) 328–333 (Scriptor, Copenhagen, 1981).
Steinmetz, M. et al. Cell 24, 125–134 (1981).
Noyes, B., Mevareck, M., Stein, R. & Agarwal, K. L. Proc. natn. Acad. Sci. U.S.A. 76, 1770–1774 (1979).
Steinmetz, M. et al. Nature 300, 35–42 (1982).
Dalbadie-McFarland, G. et al. Proc. natn. Acad. Sci. U.S.A. 79, 6409–6413 (1982).
Walter, G., Scheidtmann, K.-H., Carbone, A., Laudano, P. & Doolittle, R. J. Proc. natn. Acad. Sci. U.S.A. 77, 5197–5200 (1980).
Sutcliffe, J. G. et al. Nature 287, 801–805 (1980).
Lerner, R. A. Nature 299, 592–596 (1982).
Steinmetz, M., Winoto, A., Minard, K. & Hood, L. Cell 28, 489–498 (1982).
O'Farell, P. H. J. biol. Chem. 250, 4007–4021 (1975).
Hamlyn, P. H., Gait, M. J. & Milstein, C. Nucleic Acids Res. 9, 4485–4494 (1981).
Messing, J., Crea, R. & Seeburg, P. H. Nucleic Acids Res. 9, 309–321 (1981).
Sanger, F., Coulson, A. R., Barrell, B. G., Smith, A. J. H. & Roe, B. J. J. molec. Biol. 143, 161–178 (1980).
Suggs, S. V., Wallace, R. B., Hirose, T., Kawashima, E. H. & Itakura, K. Proc. natn. Acad. Sci. U.S.A. 78, 6613–6617 (1981).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Hunkapiller, M., Kent, S., Caruthers, M. et al. A microchemical facility for the analysis and synthesis of genes and proteins. Nature 310, 105–111 (1984). https://doi.org/10.1038/310105a0
Issue Date:
DOI: https://doi.org/10.1038/310105a0
This article is cited by
-
POSaM: a fast, flexible, open-source, inkjet oligonucleotide synthesizer and microarrayer
Genome Biology (2004)
-
Quantitative proteome analysis by solid-phase isotope tagging and mass spectrometry
Nature Biotechnology (2002)
-
Femtomole sequencing of proteins from polyacrylamide gels by nano-electrospray mass spectrometry
Nature (1996)
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.
