Nature 309, 255 - 256 (17 May 1984); doi:10.1038/309255a0

Formation of germ-line chimaeras from embryo-derived teratocarcinoma cell lines

Allan Bradley^*, Martin Evans^*, Matthew H. Kaufman^† & Elizabeth Robertson^*

^*Department of Genetics and ^†Department of Anatomy, University of Cambridge, Downing Street, Cambridge CB2 3EH, UK

The recent availability in culture of embryo-derived pluripotential cells which exhibit both a normal karyotype and a high differentiative ability¹⁻³ has encouraged us to assess the potential of these cells to form functional germ cells following their incorporation into chimaeric mice. We report here the results of blastocyst injection studies using three independently isolated XY embryo-derived cell lines (EK.CP1, EK.CC1.1 and EKCC1.2) which produce a very high proportion (>50%) of live-born animals that are overtly chimaeric. Seven chimaeric male mice, derived from these three lines, have, so far, proved to be functional germ-line chimaeras.

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