Abstract
Moloney marine leukaemia virus (M-MuLV) infection of embryonal carcinoma (EC) cells results in the integration of proviral DNA into the host cell genome1–3, but not in virus production1–6. One suggested explanation for the lack of viral gene expression in EC cells has been methylation of the integrated viral DNA1. However, subsequent reports indicated that integration of the M-MuLV DNA occurs soon after infection, but that viral DNA methylation occurs considerably later2,3. Nevertheless, viral gene expression is not observed even at early times. One possible explanation is that certain M-MuLV regulatory sequences do not function in EC cells. We now present evidence which supports this hypothesis.
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Linney, E., Davis, B., Overhauser, J. et al. Non-function of a Moloney murine leukaemia virus regulatory sequence in F9 embryonal carcinoma cells. Nature 308, 470–472 (1984). https://doi.org/10.1038/308470a0
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DOI: https://doi.org/10.1038/308470a0
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