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Article
Nature 308, 237 - 241 (15 March 1984); doi:10.1038/308237a0

Identifier sequences are transcribed specifically in brain

J. Gregor Sutcliffe, Robert J. Milner, Joel M. Gottesfeld & Richard A. Lerner

Research Institute of Scripps Clinic, 10666 North Torrey Pines Road, La Jolla, California 92037, USA

‘Identifier’ or ID sequences are present in 62% of the RNA polymerase II and III transcripts made in vitro from brain nuclei but in fewer than 4% of the transcripts made from the nuclei of other tissues. An homologous 160-nucleotide cytoplasmic poly(A)+ RNA species, BC1, and a smaller species, BC2, are located in vivo exclusively in neural tissues. Cloned ID sequences are polymerase III templates in vitro. Our data suggest a model in which brain-specific polymerase III transcription of ID sequences located in introns of brain genes activates those genes in a primary manner for polymerase II transcription.

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