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Article
Nature 307, 604 - 608 (16 February 1984); doi:10.1038/307604a0

Expression of functional acetylcholine receptor from cloned cDNAs

Masayoshi Mishina*, Tomohiro Kurosaki*, Takamasa Tobimatsu*, Yuuki Morimoto*, Masaharu Noda*, Tokuo Yamamoto*, Mineko Terao*, Jon Lindstrom, Tomoyuki Takahashi, Motoy Kuno & Shosaku Numa*

Departments of *Medical Chemistry and Physiology, Kyoto University Faculty of Medicine, Yoshida, Kyoto 606, Japan
The Salk Institute for Biological Studies, San Diego, California 92138, USA

The cloned cDNAs encoding the four subunits of the Torpedo californica acetylcholine receptor, each carried by a simian virus 40 vector, direct the synthesis of the functional receptor in a combined expression system consisting of COS monkey cells and Xenopus oocytes. Our results suggest that all four subunits are required to elicit a normal nicotinic response to acetylcholine, whereas only the α-subunit is indispensable for α-bungarotoxin binding activity.

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