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Expression of functional acetylcholine receptor from cloned cDNAs

Nature volume 307, pages 604–608 (1984)Cite this article

Abstract

The cloned cDNAs encoding the four subunits of the Torpedo californica acetylcholine receptor, each carried by a simian virus 40 vector, direct the synthesis of the functional receptor in a combined expression system consisting of COS monkey cells and Xenopus oocytes. Our results suggest that all four subunits are required to elicit a normal nicotinic response to acetylcholine, whereas only the α-subunit is indispensable for α-bungarotoxin binding activity.

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Authors and Affiliations

  1. Department of Medical Chemistry, Kyoto University Faculty of Medicine, Yoshida, Kyoto, 606, Japan

    Masayoshi Mishina, Tomohiro Kurosaki, Takamasa Tobimatsu, Yuuki Morimoto, Masaharu Noda, Tokuo Yamamoto, Mineko Terao & Shosaku Numa

  2. Department of Physiology, Kyoto University Faculty of Medicine, Yoshida, Kyoto, 606, Japan

    Tomoyuki Takahashi & Motoy Kuno

  3. The Salk Institute for Biological Studies, San Diego, California, 92138, USA

    Jon Lindstrom

Authors
  1. Masayoshi Mishina
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  2. Tomohiro Kurosaki
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  3. Takamasa Tobimatsu
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  4. Yuuki Morimoto
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  5. Masaharu Noda
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  11. Shosaku Numa
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Mishina, M., Kurosaki, T., Tobimatsu, T. et al. Expression of functional acetylcholine receptor from cloned cDNAs. Nature 307, 604–608 (1984). https://doi.org/10.1038/307604a0

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  • DOI: https://doi.org/10.1038/307604a0

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