Nature Publishing Group, publisher of Nature, and other science journals and reference works
Nature
my account e-alerts subscribe register
   
Friday 22 September 2017
Journal Home
Current Issue
AOP
Archive
Download PDF
References
Export citation
Export references
Send to a friend
More articles like this

Article
Nature 305, 189 - 193 (15 September 1983); doi:10.1038/305189a0

Construction of artificial chromosomes in yeast

Andrew W. Murray* & Jack W. Szostak*

*Dana–Farber Cancer Institute, 44 Binney St, Boston, Massachusetts 02115, USA
Program in Cell and Developmental Biology and Department of Biological Chemistry, Harvard Medical School, Boston, Massachusetts 02115, USA

Fifty-five-kilobase long artificial chromosomes containing cloned genes, replicators, centromeres and telomeres have been constructed in yeast. These molecules have many of the properties of natural yeast chromosomes. Centromere function is impaired on short (less than 20 kilobases) artificial chromosomes.

------------------

References

1. McClintock, B. Genetics 23, 315−376 (1938).
2. McClintock, B. Genetics 26, 234−282 (1941).
3. Novitski, E., Grace, D. & Strommen, C. Genetics 98, 257−273 (1981). | PubMed | ISI | ChemPort |
4. Struhl, K. & Davis, R. W. Proc. natn. Acad. Sci. U.S.A. 74, 5255−5259 (1977). | ChemPort |
5. Stinchcomb, D. T., Struhl, K. & Davis, R. W. Nature 282, 39−43 (1979). | PubMed | ISI | ChemPort |
6. Hsiao, C-L. & Carbon, J. Proc. natn. Acad. Sci. U.S.A. 76, 3829−3833 (1979). | ChemPort |
7. Clarke, L. & Carbon, J. Nature 287, 504−509 (1980). | PubMed | ISI | ChemPort |
8. Stinchcomb, D. T., Mann, C. & Davis, R. W. J. molec. Biol. 158, 157−179 (1982). | PubMed | ISI | ChemPort |
9. Szostak, J. W. & Blackburn, E. H. Cell 29, 245−255 (1982). | Article | PubMed | ISI | ChemPort |
10. Fitzgerald-Haves, M., Clarke, L. & Carbon, J. Cell 29, 235−244 (1982). | Article | PubMed | ChemPort |
11. Murray, A. W. & Szostak, J. W. Cell (in the press).
12. Blackburn, E. H. & Gall, J. G. J. molec. Biol. 120, 33−53 (1978). | PubMed | ISI | ChemPort |
13. Kiss, G. B., Amin, A. A. & Perlman, R. E. Molec. cell. Biol. 1, 535−543 (1981). | PubMed | ISI | ChemPort |
14. Campbell, D. A. & Fogel, S. Genetics 79, 383−396 (1975). | PubMed | ISI | ChemPort |
15. Liras, P., McCusker, J., Mascioli, S. & Haber, J. E. Genetics 88, 650−671 (1978).
16. Hartwell, L. H., Dutcher, S. K., Wood, J. S. & Garvik, B. Recent Adv. Yeast molec. Biol. 1, 28−38 (1982).
17. Szostak, J. W. Cold Spring Harb. Symp. quant. Biol. 47, 1187−1194 (1983). | PubMed | ISI |
18. Southern, E. M. J. molec. Biol. 98, 503−517 (1975). | PubMed | ISI | ChemPort |
19. Orr-Weaver, T. L., Szostak, J. W. & Rothstein, R. J. Proc. natn. Acad. Sci. U.S.A. 78, 6354−6358 (1981). | ChemPort |
20. Dani, G. M. & Zakian, V. A. Proc. natn. Acad. Sci. U.S.A. 80, 3406−3410 (1983). | ChemPort |
21. Bloom, K. S. & Carbon, J. Cell 29, 305−317 (1982). | Article | PubMed | ISI | ChemPort |
22. Blackburn, E. H. & Chiou, S. S. Proc. natn. Acad. Sci. U.S.A. 78, 2263−2267 (1981). | ChemPort |
23. Strathern, J. N., Newlon, C. S., Herskowitz, I. & Hicks, J. B. Cell 18, 309−319 (1979). | Article | PubMed | ISI | ChemPort |
24. Struhl, K. & Davis, R. W. J. molec. Biol. 136, 291−30 (1980).
25. Rothstein, R. J. Meth. Enzym. 101, 202−211 (1983). | Article | PubMed | ISI | ChemPort |
26. Bruenn, J. & Mortimer, R. K. J. Bact. 102, 548−555 (1970). | PubMed | ISI | ChemPort |
27. Fitzgerald-Hayes, M., Buhler, J. M., Cooper, T. G. & Carbon, J. J. Molec. cell. Biol. 2, 82−87 (1982). | ChemPort |
28. Dutcher, S. K. Molec. cell. Biol. 1, 245−253 (1981). | PubMed | ISI | ChemPort |
29. Mortimer, R. K. & Schild, D. Microbiol. Rev. 44, 519−571 (1980). | PubMed | ISI | ChemPort |
30. Agard, D. A. & Sedat, J. W. Nature 302, 676−681 (1983). | PubMed | ISI | ChemPort |
31. Sundin, O. & Varshavsky, A. Cell 21, 103−114 (1980). | Article | PubMed | ISI | ChemPort |
32. Baker, B., Carpenter, A., Esposito, M., Esposito, R. E. & Sandler, L. A. Rev. Genet. 10, 53−134 (1976). | Article | ChemPort |
33. Malone, R. E. & Easton-Esposito, R. Molec. cell. Biol. 1, 891−901 (1981). | PubMed | ISI | ChemPort |
34. Orr-Weaver, T. L., Szostak, J. W. & Rothstein, R. J. Meth. Enzym. 101, 228−245 (1983). | PubMed | ChemPort |
35. Sherman, F., Fink, G. R. & Lawrence, C. Methods in Yeast Genetics (Cold Spring Harbor Laboratory, New York, 1979).
36. Holmes, D. S. & Quigley, M. Analyt. Biochem. 114, 193−197 (1981). | PubMed | ISI | ChemPort |



© 1983 Nature Publishing Group
Privacy Policy