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Article
Nature 303, 31 - 35 (05 May 1983); doi:10.1038/303031a0

Movement of myosin-coated fluorescent beads on actin cables in vitro

Michael P. Sheetz* & James A. Spudich

Department of Structural Biology, Sherman Fairchild Building, Stanford University School of Medicine, Stanford, California 94305, USA
*Department of Physiology, University of Connecticut Health Center, Farmington, Connecticut 06032, USA

Myosin-coated fluorescent beads are observed to move unidirectionally along organized actin filament arrays in the alga, Nitella, with an average velocity similar to in vivo rates of movement in muscle and other cells. The myosin produces the motive force, as the movement is ATP dependent and is blocked by inactivation of the myosin heads. The movement, which occurs in the absence of bipolar thick filaments, provides a quantitative assay for myosin motility.

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