Abstract
A functional deficiency of factor IX, one of the coagulation factors involved in blood clotting1, leads to the bleeding disorder known as Christmas disease2, or haemophilia B. Both this disease and haemophilia A (factor VIII (C) deficiency) are X chromosome-linked and together occur at a frequency of ∼1 in 10,000 males. The molecular basis for the functional alteration of factor IX in Christmas disease is not clearly understood. As a first step towards the elucidation of the molecular events involved, we have attempted molecular cloning of the factor IX gene. We used a bovine factor IX cDNA clone, isolated using synthetic oligonucleotides as probes, to screen a cloned human gene library. Here we report the isolation and partial characterization of a λ recombinant phage containing the human factor IX gene.
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Choo, K., Gould, K., Rees, D. et al. Molecular cloning of the gene for human anti-haemophilic factor IX. Nature 299, 178–180 (1982). https://doi.org/10.1038/299178a0
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DOI: https://doi.org/10.1038/299178a0
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