Nature Publishing Group, publisher of Nature, and other science journals and reference works
Nature
my account e-alerts subscribe register
   
Wednesday 26 July 2017
Journal Home
Current Issue
AOP
Archive
Download PDF
References
Export citation
Export references
Send to a friend
More articles like this

Article
Nature 299, 32 - 36 (02 September 1982); doi:10.1038/299032a0

Sequence specificity of trinucleoside diphosphate binding to polymerized tobacco mosaic virus protein

John J. Steckert & Todd M. Schuster

Biochemistry and Biophysics Section, Biological Sciences Group, University of Connecticut, Storrs, Connecticut 06268, USA

The binding of trinucleoside diphosphates to long helical rods of tobacco mosaic virus (TMV) protein is shown to depend on base sequence, 5′ AAG 3′ binding being the strongest of the 25 trinucleoside diphosphate sequences measured. As TMV has a stoichiometry of three nucleotides per protein subunit, the sequence of TMV RNA suggested to be the nucleation site for self-assembly of the virus has three possible binding frames. From our binding constant data the most likely frame is predicted and shown to have two contiguous AAG sequences in a hairpin loop region.

------------------

References

1. Zimmern, D. & Wilson, T. M. A. FEBS Lett. 71, 294−298 (1976). | Article | PubMed | ISI | ChemPort |
2. Lebeurier, G., Nicolaieff, A. & Richards, K. E. Proc. natn. Acad. Sci. U.S.A. 74, 149−153 (1977). | ChemPort |
3. Otsuki, Y., Takebe, I., Ohno, T., Fukuda, M. & Okada, Y. Proc. natn. Acad. Sci. U.S.A. 74, 1913−1917 (1977). | ChemPort |
4. Zimmern, D. & Butler, P. J. G. Cell 11, 455−462 (1977). | Article | PubMed | ISI | ChemPort |
5. Zimmern, D. Cell 11, 463−482 (1977). | Article | PubMed | ISI | ChemPort |
6. Jonard, G., Richards, K. E., Guilley, H. & Hirth, L. Cell 11, 483−493 (1977). | Article | PubMed | ISI | ChemPort |
7. Butler, P. J. G. et al. in Structure−Function Relationships of Proteins (eds Markham, R. & Horne, R. W.) 101−110 (North-Holland, Amsterdam, 1976). | ChemPort |
8. Durham, A. C. H., Finch, J. T. & Klug, A. Nature new Biol. 229, 37−42 (1971). | PubMed | ISI | ChemPort |
9. Schuster, T. M. et al. Biophys. J. 32, 313−329 (1980). | PubMed | ISI | ChemPort |
10. Schuster, T. M., Scheele, R. B. & Khairallah, L. H. J. molec. Biol. 127, 461−485 (1979). | PubMed | ISI | ChemPort |
11. Stubbs, G., Warren, S. & Holmes, K. Nature 267, 216−221 (1977). | PubMed | ISI | ChemPort |
12. Caspar, D. L. D. Adv. Protein Chem. 18, 37−121 (1963). | PubMed | ISI | ChemPort |
13. Scheele, R. B. & Lauffer, M. A. Biochemistry 6, 3076−3081 (1967). | PubMed | ISI | ChemPort |
14. Butler, P. J. G., Durham, A. C. H. & Klug, A. J. molec. Biol. 72, 1−18 (1972). | PubMed | ISI | ChemPort |
15. Scheele, R. B. & Schuster, T. M. J. molec. Biol. 94, 519−525 (1975). | PubMed | ISI | ChemPort |
16. Durham, A. C. H., Vogel, D. & DeMarcillac, G. D. Eur. J. Biochem. 79, 151−159 (1977). | PubMed | ISI | ChemPort |
17. Shalaby, R. A. F. & Lauffer, M. A. J. molec. Biol. 116, 709−725 (1977). | PubMed | ISI | ChemPort |
18. Bloomer, A. C., Champness, J. N., Bricogne, G., Staden, R. & Klug, A. Nature 276, 362−368 (1978). | ISI | ChemPort |
19. Stubbs, G., Warren, S. & Mandelkow, E. J. supramolec. Struct. 12, 177−183 (1979). | ISI | ChemPort |
20. Mandelkow, E., Stubbs, G. & Warren, S. J. molec. Biol. 152, 375−386 (1981). | PubMed | ISI | ChemPort |
21. Schramm, G. Z. Naturf. 2b, 112−121, 249−257 (1947). | ISI |
22. Durham, A. C. H. & Klug, A. Nature new Biol. 229, 42−46 (1971). | PubMed | ISI | ChemPort |
23. Franklin, R. E. Biochim. biophys. Acta 18, 313−314 (1955). | Article | PubMed | ISI | ChemPort |
24. Guilley, H., Jonard, G., Richards, K. E. & Hirth, L. Phil. Trans. R. Soc. B276, 181−188 (1976). | ISI | ChemPort |
25. Graham, J. & Butler, P. J. G. Eur. J. Biochem. 93, 333−337 (1979). | PubMed | ISI | ChemPort |
26. Butler, P. J. G. & Lomonossoff, G. P. Biophys. J. 32, 295−312 (1980). | PubMed | ISI | ChemPort |
27. Bloomer, A. C., Graham, J., Hovmoller, S., Butler, P. J. G. & Klug, A. in Structural Aspects of Recognition and Assembly in Biological Macromolecules (eds Balaban, M., Sussman, J. L., Traub, W. & Yonath, A.) 851−864 (Balaban ISS, Rehovot, 1981). | ChemPort |
28. Stubbs, G. & Stauffacher, C. J. molec. Biol. 152, 387−396 (1981). | PubMed | ISI | ChemPort |
29. Tyulkina, L. G. et al. Virology 63, 15−29 (1975). | Article | PubMed | ISI | ChemPort |
30. Guilley, H., Jonard, G., Kukla, B. & Richards, K. E. Nucleic Acids Res. 6, 1287−1308 (1979). | PubMed | ISI | ChemPort |
31. Shire, S. J., Steckert, J. J. & Schuster, T. M. J. molec. Biol. 127, 487−506 (1979). | PubMed | ISI | ChemPort |
32. Uhlenbeck, O. C. J. molec. Biol. 65, 25−41 (1972). | PubMed | ISI | ChemPort |
33. Fraenkel-Conrat, H. & Williams, R. C. Proc. natn. Acad. Sci. U.S.A. 41, 690−698 (1955). | ChemPort |
34. Tsugita, A. et al. Proc. natn. Acad. Sci. U.S.A. 46, 1463−1469 (1960). | ChemPort |
35. Thach, R. E. & Doty, P. Science 148, 632−634 (1965). | PubMed | ISI | ChemPort |
36. Boedtker, H. Meth. Enzym. 12B, 429−458 (1968). | Article | ChemPort |
37. Shire, S. J., Steckert, J. J., Adams, M. L. & Schuster, T. M. Proc. natn. Acad. Sci. U.S.A. 76, 2745−2749 (1979). | ChemPort |



© 1982 Nature Publishing Group
Privacy Policy