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T24 human bladder carcinoma oncogene is an activated form of the normal human homologue of BALB- and Harvey-MSV transforming genes

Nature volume 298, pages 343–347 (1982)Cite this article

Abstract

A transforming gene isolated from T24 human bladder carcinoma cells is closely related to the BALB murine sarcoma virus (MSV) onc gene (v-bas). This transforming gene is localized to a 4.6 kilobase pair (kbp) region and is expressed as a 1.2-kbp polyadenylated transcript, which contains v-bas related sequences. Moreover, antisera known to detect the immunologically related onc gene products of BALB- and Harvey-MSVs recognized elevated levels of a related protein in T24 cells. The normal human homologue of v-bas was found to be indistinguishable from the T24 oncogene by heteroduplex and restriction enzyme analysis. These results imply that rather subtle genetic alterations have led to the activation of the normal human homologue of v-bas as a human transforming gene.

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Authors and Affiliations

  1. Laboratory of Cellular and Molecular Biology, National Cancer Institute, National Institutes of Health, Bethesda, Maryland, 20205, USA

    Eugenio Santos, Steven R. Tronick, Stuart A. Aaronson, Simonetta Pulciani & Mariano Barbacid

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  1. Eugenio Santos
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  2. Steven R. Tronick
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  3. Stuart A. Aaronson
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  4. Simonetta Pulciani
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  5. Mariano Barbacid
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Santos, E., Tronick, S., Aaronson, S. et al. T24 human bladder carcinoma oncogene is an activated form of the normal human homologue of BALB- and Harvey-MSV transforming genes. Nature 298, 343–347 (1982). https://doi.org/10.1038/298343a0

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