Abstract
The transcriptionally active ev-3 and inactive ev-1 endogenous retrovirus loci in chick cells differ in that ev-3 is undermethylated, preferentially sensitive to DNase I digestion, and contains nuclease hypersensitive sites in each of its two long terminal repeats. Transient exposure of cells to 5-azacytidine, a cytosine analogue which cannot be methylated at the 5 position, results in the hypomethylation and transcriptional activation of ev-1, as well as the acquisition of at least one nuclease-hypersensitive site within the chromosomal domain of ev-1.
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References
Weintraub, H. & Groudine, M. Science 93, 848–858 (1976).
Garel, A., Zolan, M. & Axel, R. Proc. natn. Acad. Sci. U.S.A. 74, 4867–4871 (1977).
Palmiter, R., McKnight, S., Mulvihill, E. & Senear, A. Cold Spring Harb. Symp. quant. Biol. 42, 639–647 (1977).
Stalder, J., Groudine, M., Dodgson, J., Engel, D. & Weintraub, H. Cell 19, 973–980 (1980).
McGhee, J. & Ginder, G. D. Nature 280, 419–420 (1979).
Mandel, J. & Chambon, P. Nucleic Acids Res. 7, 2081–2103 (1979).
Kuo, T., Mandel, J. & Chambon, P. Nucleic Acids Res. 7, 2105–2113 (1979).
Bird, A. P., Taggart, M. H. & Smith, B. A. Cell 17, 889–901 (1979).
Van der Ploag, J. & Flavell, R. Cell 19, 947–958 (1980).
Shen, S. & Maniatis, T. Proc. natn. Acad. Sci. U.S.A. 77, 6634–6638 (1980).
Weintraub, H., Larsen, A. & Groudine, M. Cell 24, 333–344 (1981).
Naveh, T. & Cedar, H. Proc. natn. Acad. Sci. U.S.A. (in the press).
Wu, C., Bingham, P. M., Livak, K., Holmgren, R. & Elgin, S. C. R. Cell 16, 797–806 (1979).
Wu, C. Nature 284, 856–860 (1980).
Stalder, J. et al. Cell 20, 451–460 (1980).
Groudine, M. & Weintraub, H. Cell 24, 393–401 (1981).
Astrin, S. Proc. natn. Acad. Sci. U.S.A. 75, 5941–5945 (1978).
Skalka, A., DeBona, P., Hishinuma, F. & McClements, W. Cold Spring Harb. Symp. quant. Biol. 44, 1097–1104 (1980).
Hughes, S., Toyoshima, K., Bishop, J. & Varmous, H. Virology 108, 189–207 (1981).
Hayward, W., Brauerman, S. & Astrin, S. Cold Spring Harb. Symp. quant. Biol. 44, 1111–1121 (1980).
Astrin, S. et al. Cold Spring Harb. Symp. quant. Biol. 44, 1105–1109 (1980).
Wang, S., Hayward, W. & Hanafusa, H. J. Virol. 24, 64–73 (1977).
Eisenman, R., Shaikh, R. & Mason, W. S. Cell 14, 889–904 (1978).
Eisenman, R., Mason, W. & Linial, M. J. Virol. 36, 62–78 (1980).
Jones, P. & Taylor, S. Cell 20, 85–93 (1980).
Groudine, M., Das, S., Neiman, P. & Weintraub, H. Cell 14, 865–878 (1978).
Ju, G. & Shalka, A. Cell 22, 379–386 (1980).
Yamamoto, T., de Crombrugghe, B. & Paston, I. Cell 22, 787–797 (1980).
Waalwyck, C. & Flavell, R. A. Nucleic Acids Res. 5, 4631–4641 (1978).
Humphries, E., Glover, C., Weiss, R. & Arrand, J. Cell 18, 803–815 (1979).
Guntaka, R., Rao, P., Mitsialis, S. & Katz, R. J. Virol. 34, 569–572 (1980).
Cohen, J. C. Cell 19, 653–662 (1980).
Akiyama, Y. & Kato, S. Biken J. 17, 105–116 (1974).
Nazerian, K. & Lee, L. J. gen. Virol. 25, 317–321 (1974).
Thomas, P. Proc. natn. Acad. Sci. U.S.A. 77, 5201–5205 (1980).
Nazerian, K., Neiman, P., Okazaki, A., Smith, E. & Crittenden, L. Avian Dis. 22, 732–741 (1978).
Varshavsky, A., Sundin, O. & Bohn, M. Cell 16, 453–466 (1979).
Weisbrod, S., Groudine, M. & Weintraub, H. Cell 19, 289–301 (1980).
Weintraub, H. Nucleic Acids Res. 8, 4745–4754 (1980).
Benoist, C. & Chambon, P. Nature 290, 304–309 (1981).
Sundin, O. & Varshavsky, A. J. molec. Biol. 132, 535–546 (1979).
Varshavsky, A., Sundin, O. & Bahn, M. Nucleic Acids Res. 5, 3469–3477 (1978).
Blair, D., Mclements, W., Oskarsson, M., Fischarger, P. & Vande Wande, G. Proc. natn. Acad. Sci. U.S.A. 77, 3504–3508 (1980).
Neel, B., Hayward, W., Robinson, H., Fang, J. & Astrin, S. Cell 23, 323–334 (1981).
Schmitz, A. & Galas, D. Nucleic Acids Res. 6, 111–137 (1979).
Weiss, R., Friis, R., Katz, E. & Vogt, P. Virology 46, 920–938 (1971).
Robinson, H., Swanson, C., Hruska, J. & Crittendon, L. Virology 69, 63–74 (1976).
Christman, J., Price, P., Pedriman, L. & Acs, G. Eur. J. Biochem. 81, 53–61 (1977).
Chiang, P., Science 211, 1164–1166 (1981).
Lowy, D. Proc. natn. Acad. Sci. U.S.A. 75, 5539–5543 (1978).
Razin, A. & Riggs, A. Science 210, 604–610 (1980).
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Groudine, M., Eisenman, R. & Weintraub, H. Chromatin structure of endogenous retroviral genes and activation by an inhibitor of DNA methylation. Nature 292, 311–317 (1981). https://doi.org/10.1038/292311a0
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DOI: https://doi.org/10.1038/292311a0
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