Nature 290, 304 - 310 (26 March 1981); doi:10.1038/290304a0

In vivo sequence requirements of the SV40 early promoter region

Christophe Benoist^* & Pierre Chambon

Laboratoire de Génétique Moléculaire des Eucaryotes du CNRS, Unité 184 de Biologie Moléculaire et de Génie Génétique de l'INSERM, Institut de Chimie Biologique, Faculté de Médecine, 67085 Strasbourg, France
^*Present address: Stanford University Medical School, Department of Genetics, Stanford, California 94305, USA.

To investigate the sequences necessary for proper initiation of transcription of SV40 early genes, we have constructed several deletion mutants in the promoter region. The TATA box region is apparently involved infixing initiation precisely within a narrow area, but is dispensable for gene expression, while the sequences located more than 150 base pairs upstream are indispensable.

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