Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

  • Letter
  • Published:

Glucagon and glicentin immunoreactivity are topologically segregated in the α granule of the human pancreatic A cell

Nature volume 284, pages 66–67 (1980)Cite this article

Abstract

Glicentin is a 100-amino acid polypeptide purified from the porcine intestine1 and containing the immunodeterminants of glucagon2. We have previously reported3 the presence of glicentin immunoreactivity in the glucagon-containing, specific secretory granules (α granules) of the pancreatic and gastric A cell. With an improved immunocytochemical probe, the protein A–gold (pAg) technique4, we are now able to show that glucagon and glicentin-like material are topologically segregated in the a granule of the human pancreatic A cell.

This is a preview of subscription content, access via your institution

Access options

Buy this article

  • Purchase on Springer Link
  • Instant access to full article PDF
Buy now

Prices may be subject to local taxes which are calculated during checkout

Similar content being viewed by others

References

  1. Sundby, F., Jacobsen, H. & Moody, A. J. Horm. Metab. Res. 8, 366–371 (1976).

    Article  CAS  Google Scholar 

  2. Jacobsen, H., Demandt, A., Moody, A. J. & Sundby, F. Biochim. biophys. Acta 493, 452–459 (1977).

    Article  CAS  Google Scholar 

  3. Ravazzola, M. et al. Endocrinology 105, 499–508 (1979).

    Article  CAS  Google Scholar 

  4. Roth, J., Bendayan, M. & Orci, L. J. Histochem. Cytochem. 26, 1074–1081 (1978).

    Article  CAS  Google Scholar 

  5. Like, A. A. Lab. Invest. 16, 937–951 (1967).

    CAS  PubMed  Google Scholar 

  6. Sternberger, L. A. in Electron Microscopy of Enzymes, Principles and Methods (ed. Hayat, M. A.) 150–181 (Van Nostrand, New York, 1973).

    Google Scholar 

  7. Bussolati, G., Capella, C., Vassallo, G. & Solcia, E. Diabetologia 7, 181–188 (1971).

    Article  CAS  Google Scholar 

  8. Grimelius, L. et al. Virchows Arch. B Cell Path. 20, 217–288 (1976).

    CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Institute of Histology and Embryology, University of Geneva Medical School, 1211, Geneva, 4, Switzerland

    Mariella Ravazzola & Lelio Orci

Authors
  1. Mariella Ravazzola
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Lelio Orci
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Ravazzola, M., Orci, L. Glucagon and glicentin immunoreactivity are topologically segregated in the α granule of the human pancreatic A cell. Nature 284, 66–67 (1980). https://doi.org/10.1038/284066a0

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1038/284066a0

This article is cited by

Comments

By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.

Search

Advanced search

Quick links

Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing