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Article
Nature 281, 646 - 650 (25 October 1979); doi:10.1038/281646a0

Nucleotide sequence of the hepatitis B virus genome (subtype ayw) cloned in E. coli

Francis Galibert*, Elisabeth Mandart*, Françoise Fitoussi*, Pierre Tiollais & Patrick Charnay*§

*Laboratoire d'Hématologie Expérimentale, Centre Hayem, Hôpital Saint-Louis, Paris, France
Recombinaison et Expression Génétique (INSERM U.163), Unité de Génie Génétique, Institut Pasteur, Paris, France
To whom correspondence should be addressed.
§Present address: Unité de Génie Génétique, Institut Pasteur, Paris, France.

The complete nucleotide sequence of hepatitis B virus genome (subtype ayw) cloned in Escherichia coli has been determined using the Maxam and Gilbert method and the dideoxynucleotide method. This sequence is 3,182 nucleotides long. Location of the nonsense codons shows that the coding capacity of the L chain is larger than the coding capacity of the Schain. Eight open regions, able to code for polypeptide chains larger than 100 amino acids, have been located. Region 6, which is the largest, covers more than 80% of the genome. The gene S which codes for polypeptide I of the Hbs Ag and was previously located between coordinates 95.1 and 73.6 is contained in region 7.

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