Nature 281, 394 - 396 (04 October 1979); doi:10.1038/281394a0

Identical 3' non-coding sequences in five mouse Ig chain mRNAs favour a unique C gene

Nicholas M. Gough, Suzanne Cory & Jerry M. Adams

Molecular Biology Laboratory, The Walter and Eliza Hall Institute of Medical Research, PO Royal Melbourne Hospital, Victoria 3050, Australia

The constant regions of immunoglobulin chains are encoded by a very small but unknown number of genes. The kinetics of nucleic acid reassociation is consistent with up to four C genes per haploid mouse genome¹⁻⁴, while saturation hybridisation suggests that there are two C genes⁵. The amino acid sequences proposed for seven C regions do differ slightly⁶⁻¹⁰, but at least some of these differences result from sequencing errors¹¹. Determining the nucleotide sequence of the C region of mRNAs¹¹ and the adjacent 3' non-coding region should more readily reveal any slight differences reflecting a multiplicity of C genes. Examination of the 3' non-coding region may be particularly useful, as this region is not constrained to encode the largely invariant C amino acid sequence. Sequences reported for the 3' terminal regions of two mRNAs, MOPC 21 (refs 11, 12) and MOPC 41A (ref. 13), were identical for the first 59 residues, but the subsequent, tentative, 45 residues proposed for MOPC 41A mRNA differed from the MOPC 21 sequence. It was unclear, however, whether these differences resulted from methodological problems, or genuine sequence divergence. We have therefore re-examined the MOPC 41A 3' terminal sequence in a direct comparison with that of MOPC 21 and also determined 3' terminal sequences of about 100 residues in three other mRNAs. We confirm here the sequence presented by Hamlyn et al. ¹¹ for the MOPC 21 mRNA and show that not only the MOPC 41A mRNA, but also the three other mRNAs have the same sequence.

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