Nature Publishing Group, publisher of Nature, and other science journals and reference works
Nature
my account e-alerts subscribe register
   
Wednesday 23 August 2017
Journal Home
Current Issue
AOP
Archive
Download PDF
References
Export citation
Export references
Send to a friend
More articles like this

Letters
Nature 279, 728 - 731 (21 June 1979); doi:10.1038/279728a0

Seminalplasmin is a potent inhibitor of E. coli RNA polymerase in vitro

K. H. SCHEIT*, E. S. P. REDDY & P. M. BHARGAVA

*Max-Planck Institut für Biophysikalische Chemie, Abeteilung Molekulare Biologie, Am Fassberg, D-3400 Göttingen-Nikolausberg, FRG
Centre for Cellular & Molecular Biology, Regional Research Laboratory, Hyderabad 500 009, India

SEMINALPLASMIN, a protein isolated from bovine seminal plasma, specifically and almost completely inhibits synthesis of rRNA in whole Escherichia coli cells at extracellular concentrations lower than those needed for bacteriocidal action of many established antibacterial agents1. We show here that seminalplasmin also strongly inhibits the transcription of various natural and synthetic templates by E. coli RNA polymerase in vitro, and that it does so by binding strongly to the polymerase. Seminalplasmin is, to our knowledge, the first protein isolated from an eukaryote to inhibit RNA polymerase. There is, however, a protein synthesised in E. coli following a phage infection and coded by the phage genome, which appears to inhibit E. coli RNA polymerase2.

------------------

References

1. Reddy, E. S. P. & Bhargava, P. M. Nature 279, 725–728 (1979).
2. Rabussay, D. & Geiduschek, E. P. in Comprehensive Virology Vol. 8 (eds Fraenkel-Conrat, H. & Wagner, R. P.) 1–196 (Plenum, New York).
3. Smagowicz, J. W. & Scheit, K. H. Nucleic Acids Res. 4, 3863–3876 (1977).
4. Faerber, P. & Scheit, K. H. Eur. J. Biochem. 50, 549–555 (1975).
5. Laemmli, U. K. Nature 227, 680–685 (1970).



© 1979 Nature Publishing Group
Privacy Policy