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Letter
Nature 277, 245-246 (18 January 1979) | doi:10.1038/277245a0; Accepted 10 November 1978
Formation of a new fluorophore on irradiation of carboxymethylated D-glyceraldehyde-3-phosphate dehydrogenase
YEN-SEN HO & CHEN-LU TSOU
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Abstract
BECAUSE of its physiological importance, allosteric properties and ready availability, D-glyceraldehyde-3-phosphate dehydrogenase (G3PDH) is one of the most thoroughly studied of the dehydrogenases. We report here a study of the ligand binding properties of G3PDH. We observed that when active site carboxymethylated G3PDH was irradiated with near ultraviolet light in the presence of NAD+, a new fluorophore was formed.
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