Nature 277, 245 - 246 (18 January 1979); doi:10.1038/277245a0

Formation of a new fluorophore on irradiation of carboxymethylated D-glyceraldehyde-3-phosphate dehydrogenase

YEN-SEN HO & CHEN-LU TSOU

Institute of Biophysics, Academia Sinica, Peking, China

BECAUSE of its physiological importance, allosteric properties and ready availability, D-glyceraldehyde-3-phosphate dehydrogenase (G3PDH) is one of the most thoroughly studied of the dehydrogenases. We report here a study of the ligand binding properties of G3PDH. We observed that when active site carboxymethylated G3PDH was irradiated with near ultraviolet light in the presence of NAD⁺, a new fluorophore was formed.

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