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Recognition sequence of specific endonuclease BamHI from Bacillus amyloliquefaciens H

Abstract

MANY specific endonucleases (restriction endonucleases) have been isolated and recognition sequences have been determined for a number of them1. The isolation of a new specific endonuclease, BamHI, from Bacillus amyloliquefaciens H has recently been described2. We have determined the recognition sequence of BamHI and find that it cleaves the two-fold rotationally symmetric sequence at the positions indicated by the arrows generating fragments with cohesive termini.

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ROBERTS, R., WILSON, G. & YOUNG, F. Recognition sequence of specific endonuclease BamHI from Bacillus amyloliquefaciens H. Nature 265, 82–84 (1977). https://doi.org/10.1038/265082a0

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