Abstract
MANY specific endonucleases (restriction endonucleases) have been isolated and recognition sequences have been determined for a number of them1. The isolation of a new specific endonuclease, BamHI, from Bacillus amyloliquefaciens H has recently been described2. We have determined the recognition sequence of BamHI and find that it cleaves the two-fold rotationally symmetric sequence at the positions indicated by the arrows generating fragments with cohesive termini.
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References
Roberts, R. J., CRC Critical Reviews in Biochemistry 4, 123–164 (1976).
Wilson, G. A., and Young, F. E., J. molec. Biol., 97, 123–125 (1975).
Richardson, C. C., Proc. natn. Acad. Sci. U.S.A., 54, 158–165 (1965).
Glynn, I. M., and Chappell, J. B., Biochem. J., 90, 147–149 (1964).
Brownlee, G. G., and Sanger, F., Eur. J. Biochem., 11, 395–399 (1969).
Jay, E., Bambara, R., Padmanabhan, R., and Wu, R., Nuc. Acids Res., 1, 331–353 (1974).
Lehman, I. R., and Nussbaum, A. L., J. biol. Chem., 239, 2628–2636 (1964).
Barrell, B. G., in Procedures in Nucleic Acid Research, vol. 2, (edit. by Cantoni, G. L., and Davies, D. R.), 751–779 (Harper and Row, New York, 1971).
Sanger, F., Donelson, J. E., Coulson, A. R., Kossel, H., and Fischer, D., Proc. natn. Acad. Sci. U.S.A., 70, 1209–1213 (1973).
Roberts, R. J., Arrand, J. R., and Keller, W., Proc. natn. Acad. Sci. U.S.A., 71, 3829–3833 (1974).
Englund, P. T., J. molec. Biol., 66, 209–224 (1972).
Richardson, C. C., Lehman, I. R., and Kornberg, A., J. biol. Chem., 239, 251–258 (1964).
Hedgpeth, J., Goodman, H. M., and Boyer, H. W., Proc. natn. Acad. Sci. U.S.A., 69, 3448–3452 (1972).
Old, R., Murray, K., and Roizes, G., J. molec. Biol., 92, 331–339 (1975).
Hamer, D. H., and Thomas, C. A., Jr, Proc. natn. Acad. Sci. U.S.A., 73, 1537–1541 (1976).
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ROBERTS, R., WILSON, G. & YOUNG, F. Recognition sequence of specific endonuclease BamHI from Bacillus amyloliquefaciens H. Nature 265, 82–84 (1977). https://doi.org/10.1038/265082a0
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DOI: https://doi.org/10.1038/265082a0
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