Abstract
MALARIA is a major cause of morbidity and mortality, claiming an estimated one million lives a year in Africa alone1. Plasmodium falciparum, the most important agent of human malaria, has not been maintained for more than 2–6 d in vitro2–10. The development of a vaccine for malaria depends on suitable culture methods for the production of relevant antigens1. We describe here a new culture method for P. falciparum, which involves an inoculum of cryopre-served parasites and make possible an analysis of merozoite–erythrocyte interactions, growth for more than 3 weeks in vitro and the collection of merozoites. Merozoites are of particular interest because vaccination with the merozoites of P. knowlesi protects rhesus monkeys from infection with this species of malaria parasite11.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Developments in Malaria Immunology, Tech. Rep. Ser. Wld Hlth Org., 579 (1975).
Diggs, C., et al., J. Parasit., 57, 187–188 (1971).
Diggs, C., Joseph, K., Flemings, B., Snodgrass, R., and Hines, F., Am. J. trop. Med. Hyg., 24, 760–763 (1975).
Mitchell, G. H., Butcher, G. A., Voller, A., and Cohen, S., Parasitology, 72, 149–162 (1976).
Pavanand, K., Permpanich, B., Chuanak, N., and Sookto, P., J. Parasit., 60, 537–539 (1974).
Phillips, R. S., Trigg, P. I., Scott-Finnigan, T. J., and Bartholomew, R. K., Parasitology, 65, 525–535 (1972).
Siddiqui, W. A., and Schnell, J. V., J. Parasit., 59, 516–519 (1973).
Trager, W., J. Protozool, 18, 239–242 (1971).
Trigg, P. I., Parasitology, 71, 433–436 (1975).
Wilson, R. J. M., and Bartholomew, R. K., Parasitology, 71, 183–192 (1975).
Mitchell, G. H., Butcher, G. A., and Cohen, S., Nature, 252, 311–313 (1974); Immunology, 29, 397–407 (1975).
Hickman, R. L., Milit. Med., 134, 741–746 (1969).
Trenholme, G. M., et al., Science, 190, 792–794 (1975).
Grant, P. T., and Fulton, J. D., Biochem. J., 66, 242–250 (1957).
Dennis, E. D., Mitchell, G. H., Butcher, G. A., and Cohen, S., Parasitology, 71, 475–481 (1975).
Williams, S. G., and Richards, W. H. G., Ann. trop. Med. Parasit., 67, 169–177 (1973).
Cohen, S., Butcher, G. A., and Crandall, R. B., Nature, 233, 368–371 (1969).
Tiner, J. D., Proc. helminth. Soc. Wash., 39, 211–219 (1972).
Butcher, G. A., Mitchell, G. H., and Cohen, S., Nature, 244, 40–42 (1973).
Miller, L. H., Mason, S. J., Dvorak, J. A., McGinniss, M. H., and Rothman, I. K., Science, 189, 561–563 (1975).
Miller, L. H., Mason, S. J., Clyde, D. F., and McGinniss, M. H., New Engl. J. Med., 295, 302–304 (1976).
Miller, L. H., Dvorak, J. A., Shiroishi, T., and Durocher, J. R., J. exp. Med., 138, 1597–1601 (1973).
Miller, L. H., Shiroishi, T., Dvorak, J. A., Durocher, J. R., and Schrier, B. K., J. molec. Med., 1, 55–63 (1975).
Trager, W., and Jensen, J. B., Science, 193, 673–675 (1976).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
HAYNES, J., DIGGS, C., HINES, F. et al. Culture of human malaria parasites Plasmodium falciparum. Nature 263, 767–769 (1976). https://doi.org/10.1038/263767a0
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1038/263767a0
This article is cited by
-
Antiplasmodial activity, structure–activity relationship and studies on the action of novel benzimidazole derivatives
Scientific Reports (2023)
-
Development and optimizing a simple and cost-effective medium for in vitro culture of Plasmodium berghei-ANKA strain with conserving its infectivity in BALB/c mice
BMC Research Notes (2022)
-
In vitro production of infectious Plasmodium falciparum sporozoites
Nature (2022)
-
A portfolio of geographically distinct laboratory-adapted Plasmodium falciparum clones with consistent infection rates in Anopheles mosquitoes
Malaria Journal (2021)
-
Robust continuous in vitro culture of the Plasmodium cynomolgi erythrocytic stages
Nature Communications (2019)
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.