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Article
Nature 260, 500 - 507 (08 April 1976); doi:10.1038/260500a0

Complete nucleotide sequence of bacteriophage MS2 RNA: primary and secondary structure of the replicase gene

W. Fiers, R. Contreras, F. Duerinck, G. Haegeman, D. Iserentant, J. Merregaert, W. Min Jou, F. Molemans, A. Raeymaekers, A. Van den Berghe, G. Volckaert & M. Ysebaert

Laboratory of Molecular Biology, University of Ghent, 9000 Ghent, Belgium

Bacteriophage MS2 RNA is 3,569 nucleotides long. The nucleotide sequence has been established for the third and last gene, which codes for the replicase protein. A secondary structure model has also been proposed. Biological properties, such as ribosome binding and codon interactions can now be discussed on a molecular basis. As the sequences for the other regions of this RNA have been published already, the complete, primary chemical structure of a viral genome has now been established.

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