Nature 259, 455 - 458 (12 February 1976); doi:10.1038/259455a0

T4 gene 32 protein model for control of activity at replication fork

Herbert Moise & Junko Hosoda

Space Sciences Laboratory, University of California, Berkeley, California 94720

Limited hydrolysis of gene 32 protein by various proteinases results in the production of three stable cleavage products. Two of these products show an affinity for native T4 DNA cellulose that the uncleaved protein does not exhibit. A model for proteolytic cleavage and for the local unwinding of DNA in advance of the replication fork is discussed in terms of this unusual binding affinity.

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