Abstract
CONVENTIONAL nonspecific precipitation methods sometimes depend on affinities which can be used in a more selective fashion by modern chromatographic techniques. The affinity of proteins for heavy metal ions, for example, may provide a basis for their purification and analysis. A highly flexible method based on such affinities is described here.
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References
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PORATH, J., CARLSSON, J., OLSSON, I. et al. Metal chelate affinity chromatography, a new approach to protein fractionation. Nature 258, 598–599 (1975). https://doi.org/10.1038/258598a0
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DOI: https://doi.org/10.1038/258598a0
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