Nature 258, 636 - 639 (18 December 1975); doi:10.1038/258636a0

Replication of human KB cell DNA after infection by adenovirus type 12

MARY M. PATER^* & STANLEY MAK

Biology Department, McMaster University, Hamilton, Ontario, Canada L8S 4K1
^*Present address: Department of Microbiology, College of Medicine, The Milton S. Hershey Medical Center, Pennsylvania State University, Hershey, Pennsylvania 17033.

LYTIC infection of human KB cells by adenovirus type 12 (ad 12) results in the early inhibition of host cell DNA synthesis¹. We have looked at some aspects of cell DNA replication after infection of KB cells by ad 12. We observed that the ligation of the Okazaki fragments is slower in cells affected by this virus. We also observed that the inhibition of cell DNA synthesis by this virus is not a random process and that certain classes of DNA, distinguished by their higher buoyant density and by their high reiteration frequency, are selectively synthesised.

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